Zhu Jia, Huang Xiaolan, Su Gaixiu, Wang Li, Wu Fengqi, Zhang Ting, Song Guowei
Graduate School of Peking Union Medical College, Division of Pediatric Rheumatology, Children's Hospital Affiliated Capital Institute of Pediatrics, Yabao Road No. 2, Chaoyang District, Beijing, Beijing, 100020, China.
Clin Rheumatol. 2014 Jun;33(6):807-15. doi: 10.1007/s10067-014-2583-5. Epub 2014 Apr 1.
Paediatric systemic lupus erythematosus (pSLE) refers to childhood-onset systemic lupus erythematosus. pSLE has its own unique characteristics, and its pathogenesis is unclear. To study the relationship between microRNAs (miRNAs) and pSLE, we selected three pSLE patients who were newly diagnosed and had not yet been treated, and two controls were also included. We collected their peripheral blood mononuclear cells to perform Agilent human miRNA (8×15 k) 12.0 analysis. To verify the results, we next selected 12 other pSLE patients who had different disease activities and 3 healthy controls and conducted real-time PCR. The results showed high expression of miRNA-516a-3p, miRNA-629 and miRNA-525-5p in pSLE patients with active disease; these levels were normal in patients without active disease. Increased expression levels of these three miRNAs were positively correlated with the score obtained from the systemic lupus erythematosus disease activity index scoring system (SLEDAI) 2000 and C-reactive protein (CRP) levels. Furthermore, the target genes of these three miRNAs were important to the pathogenesis of pSLE. Therefore, these three miRNAs might be specific to pSLE and may be used as novel biomarkers of pSLE to diagnose and monitor the disease.
儿童系统性红斑狼疮（pSLE）是指儿童期发病的系统性红斑狼疮。pSLE有其自身独特的特征，其发病机制尚不清楚。为了研究微小RNA（miRNA）与pSLE之间的关系，我们选取了3例新诊断且尚未接受治疗的pSLE患者，并纳入了2例对照。我们收集他们的外周血单个核细胞进行安捷伦人类miRNA（8×15 k）12.0分析。为了验证结果，接下来我们选取了另外12例具有不同疾病活动度的pSLE患者和3例健康对照进行实时聚合酶链反应。结果显示，疾病活动期的pSLE患者中miRNA - 516a - 3p、miRNA - 629和miRNA - 525 - 5p表达上调；在无疾病活动的患者中这些水平正常。这三种miRNA表达水平的升高与系统性红斑狼疮疾病活动指数评分系统（SLEDAI）2000评分及C反应蛋白（CRP）水平呈正相关。此外，这三种miRNA的靶基因对pSLE的发病机制很重要。因此，这三种miRNA可能是pSLE特有的，可作为pSLE诊断和监测疾病的新型生物标志物。
