Research Center for Pharmaceutical Nanotechnology and Department of Endodontics, Tabriz University (Medical Sciences), Tabriz, Iran.
J Endod. 2012 Oct;38(10):1391-4. doi: 10.1016/j.joen.2012.06.027. Epub 2012 Jul 25.
The aim of this study was to evaluate the effect of MTAD as final rinse on removal of the smear layer subsequent to primary irrigation with 1.3% sodium hypochlorite (NaOCl) during 10-minute instrumentation periods.
Forty teeth were divided into 2 experimental groups, each containing 15 teeth, and 1 positive control group of 10 teeth. The canals were prepared with hand and rotary instruments. In the MTAD group, the root canals were flushed with 1.3% NaOCl solution during 10-minute instrumentation, and MTAD was used as the final rinse. In other experimental group, 5.25% NaOCl was used during instrumentation, and 17% ethylenediaminetetraacetic acid (EDTA) was used as the final rinse. In the positive control group, sterile distilled water was used for irrigation. The overall instrumentation period for each canal was 10 minutes. The amount of the smear layer and degree of erosion qualified according to the study by Torabinejad et al by using a scanning electron microscope. Data were analyzed by Kruskal-Wallis and Mann-Whitney tests. All statistical analyses were set with a significance level of α = 0.05.
In the positive control group, debris and heavy smear layer covered the root canal surface and the tubules. In MTAD group, moderate smear layer covered the root canal surface and the tubules. In EDTA group, there was no smear layer on surface of canals. There were significant differences between groups (α < 0.0001). Mann-Whitney test showed significant difference between experimental groups (α = 0.01).
In 10-minute instrumentation period, the use of 5.25% NaOCl during instrumentation and 17% EDTA as the final rinse is more effective than using 1.3% NaOCl as primary irrigation and MTAD as final rinse on removal of the smear layer.
本研究旨在评估在 10 分钟器械预备期间，使用 1.3%次氯酸钠（NaOCl）作为初始冲洗液后，MTAD 作为终末冲洗液对去除玷污层的效果。
将 40 颗牙齿分为 2 个实验组，每个组包含 15 颗牙齿，以及 1 个阳性对照组，包含 10 颗牙齿。使用手动和旋转器械对根管进行预备。在 MTAD 组中，在 10 分钟器械预备期间用 1.3% NaOCl 溶液冲洗根管，并用 MTAD 作为终末冲洗液。在另一个实验组中，在器械预备期间使用 5.25% NaOCl，用 17%乙二胺四乙酸（EDTA）作为终末冲洗液。在阳性对照组中，用无菌蒸馏水冲洗。每个根管的总体器械预备时间为 10 分钟。根据 Torabinejad 等人的研究，使用扫描电子显微镜对玷污层的量和侵蚀程度进行评估。使用 Kruskal-Wallis 和 Mann-Whitney 检验对数据进行分析。所有统计分析的显著性水平均设为 α = 0.05。
在阳性对照组中，根管表面和小管内覆盖着大量的碎屑和厚重的玷污层。在 MTAD 组中，根管表面和小管内覆盖着中度的玷污层。在 EDTA 组中，根管表面没有玷污层。各组之间有显著差异（α < 0.0001）。Mann-Whitney 检验显示实验组之间有显著差异（α = 0.01）。
在 10 分钟器械预备期间，使用 5.25% NaOCl 作为器械预备期间的冲洗液，用 17% EDTA 作为终末冲洗液，比使用 1.3% NaOCl 作为初始冲洗液，用 MTAD 作为终末冲洗液更有效地去除玷污层。
