Zand Vahid, Mokhtari Hadi, Reyhani Mohammad-Frough, Nahavandizadeh Neda, Azimi Shahram
Assistant Professor, Department of Endodontics, Faculty of Dentistry, Tabriz University of Medical Sciences, Tabriz, Iran.
J Clin Exp Dent. 2017 Nov 1;9(11):e1310-e1314. doi: 10.4317/jced.54179. eCollection 2017 Nov.
The aim of the present study was to compare the amount of the smear layer remaining in prepared root canals with different protocols of Bio RaCe files and XP-endo Finisher file (XPF) in association with 17% EDTA and sodium hypochlorite solution.
A total of 68 extracted single-rooted teeth were randomly divided into 4 experimental groups (n=14) and two control groups (n=6). The root canals were prepared with Bio RaCe files (FKG Dentaire, Switzerland) using the crown-down technique based on manufacturer's instructions and irrigated according to the following irrigation techniques: Group 1: XPF with 2 mL of 2.5% NaOCl for 1 minute. Group 2:, XPF with 1 mL of 17% EDTA for one minute. Group 3: XPF was used for 1 minute in association with normal saline solution. Group 4: XP-endo Finisher file for 30 seconds in association with 2.5% NaOCl and 17% EDTA for 30 seconds. The negative control group: NaOCl (2.5%) was used during root canal preparation, followed by irrigation with 17% EDTA at the end of root canal preparation. The positive control group: Normal saline solution was used for irrigation during root canal preparation. In all the groups, during preparation of the root canals with Bio RaCe file, 20 mL of 2.5% NaOCl was used for root canal irrigation and at the end of the procedural steps 20 mL of normal saline solution was used as a final irrigant. The samples were analyzed under SEM at ×1000‒2000 magnification and evaluated using Torabinejad scoring system. Data were analyzed with non-parametric Kruskal-Wallis test and post hoc Mann-Whitney U test, using SPSS. Statistical significant was defined at <0.05.
The results of the study showed the least amount of the smear layer at coronal, middle and apical thirds of the root canals in groups 2, which was not significantly different from the negative control group (<0.5).
Under the limitations of the present study, use of a combination of NaOCl and EDTA in association with XPF exhibited the best efficacy for the removal of the smear layer. Smear layer, XP-endo Finisher file, EDTA, Sodium hypochlorite.
本研究的目的是比较使用不同方案的Bio RaCe锉和XP-endo Finisher锉（XPF）联合17%乙二胺四乙酸（EDTA）和次氯酸钠溶液时，预备根管内残留的玷污层量。
总共68颗拔除的单根牙被随机分为4个试验组（n = 14）和2个对照组（n = 6）。根据制造商的说明，采用冠向下技术用Bio RaCe锉（瑞士FKG Dentaire公司）预备根管，并按照以下冲洗技术进行冲洗：第1组：XPF联合2 mL 2.5%次氯酸钠冲洗1分钟。第2组：XPF联合1 mL 17% EDTA冲洗1分钟。第3组：XPF联合生理盐水冲洗1分钟。第4组：XP-endo Finisher锉使用30秒，联合2.5%次氯酸钠和17% EDTA各冲洗30秒。阴性对照组：根管预备期间使用2.5%次氯酸钠，根管预备结束时用17% EDTA冲洗。阳性对照组：根管预备期间用生理盐水冲洗。在所有组中，使用Bio RaCe锉预备根管时，用20 mL 2.5%次氯酸钠冲洗根管，操作步骤结束时用20 mL生理盐水作为最终冲洗液。在扫描电子显微镜下以×1000 - 2000倍放大率对样本进行分析，并使用Torabinejad评分系统进行评估。使用SPSS软件，采用非参数Kruskal-Wallis检验和事后Mann-Whitney U检验对数据进行分析。统计学显著性定义为P < 0.05。
研究结果显示，第2组根管冠部、中部和根尖三分之一处的玷污层量最少，与阴性对照组无显著差异（P < 0.5）。
在本研究的局限性下，次氯酸钠和EDTA联合XPF使用对去除玷污层显示出最佳效果。玷污层、XP-endo Finisher锉、EDTA、次氯酸钠
