Danert Fátima C, Zampini Catiana, Ordoñez Roxana, Maldonado Luis, Bedascarrasbure Enrique, Isla María Inés
Nat Prod Commun. 2014 Feb;9(2):167-70.
Bee propolis is a natural product extensively used as an ingredient in functional foods in amounts that may confer health benefits. The aim of this study was to investigate the nutritional and functional properties (antioxidant activity) of aqueous and ethanolic extracts of propolis samples from Santiago del Estero province, northern Argentina. All propolis extracts contained macronutrients (glucose, fructose, sucrose and proteins), micronutrients (Na, K, Ca, P and Mg) and trace elements (Fe). Spectrophotometric, TLC and HPLC-DAD analyses showed the presence of several phenolic, flavonoid and non-flavonoid compounds, but in all cases the flavonoids prevailed. The PCA of polyphenolic content provided a clear separation of propolis in Group 1 (SE1, 2, 3, 4 and 7) and Group 2 (SE5 and 6) associated with phenolic compound content and collection regions. Two compounds, pinocembrin and chrysin, which could be used as chemical markers of Santiago del Estero propolis, were identified in all samples. Propolis samples extracted with water presented better radical scavenging ability than ethanolic extracts, independent of the antioxidant method (scavenging activity of ABTS*+, DPPH*, HO* and O2(-)* and beta-carotene bleaching test). Such results correlated closely with the levels of total phenols and flavonoids in samples. The results justify the use of Argentine propolis as a functional dietary supplement.
蜂蜡是一种天然产物，被广泛用作功能性食品的成分，其用量可能带来健康益处。本研究的目的是调查阿根廷北部圣地亚哥 - 德尔埃斯特罗省蜂胶样品的水提取物和乙醇提取物的营养及功能特性（抗氧化活性）。所有蜂胶提取物都含有常量营养素（葡萄糖、果糖、蔗糖和蛋白质）、微量营养素（钠、钾、钙、磷和镁）以及微量元素（铁）。分光光度法、薄层色谱法和高效液相色谱 - 二极管阵列检测分析表明存在多种酚类、黄酮类和非黄酮类化合物，但在所有情况下黄酮类化合物占主导。多酚含量的主成分分析清楚地将蜂胶分为第1组（SE1、2、3、4和7）和第2组（SE5和6），这与酚类化合物含量和采集区域有关。在所有样品中都鉴定出两种化合物，松属素和白杨素，它们可作为圣地亚哥 - 德尔埃斯特罗蜂胶的化学标志物。用水提取的蜂胶样品比乙醇提取物具有更好的自由基清除能力，这与抗氧化方法（ABTS* +、DPPH*、HO*和O2(-)*的清除活性以及β - 胡萝卜素漂白试验）无关。这些结果与样品中总酚和黄酮类化合物的含量密切相关。这些结果证明了阿根廷蜂胶可作为功能性膳食补充剂使用。
