Foord Adam J, Boyd Victoria, White John R, Williams David T, Colling Axel, Heine Hans G
CSIRO Australian Animal Health Laboratory, Division of Animal, Food and Health Sciences, Geelong, Vic., Australia.
CSIRO Australian Animal Health Laboratory, Division of Animal, Food and Health Sciences, Geelong, Vic., Australia.
J Virol Methods. 2014 Jul;203:65-72. doi: 10.1016/j.jviromet.2014.03.018. Epub 2014 Mar 30.
Flaviviruses of the Japanese encephalitis virus (JEV) serocomplex include major human and animal pathogens that have a propensity to spread and emerge in new geographic areas. Different genotypes or genetic lineages have been defined for many of these viruses, and they are distributed worldwide. Tools enabling rapid detection of new or emerging flaviviruses and differentiation of important subgroups have widespread application for arbovirus diagnosis and surveillance, and are crucial for detecting virus incursions, tracking virus emergence and for disease control. A microsphere suspension array assay was developed to identify JEV serocomplex flaviviruses of medical and veterinary importance. Assay performance was evaluated using representative virus strains as well as clinical and surveillance samples. The assay detected all JEV serocomplex viruses tested in this study with an apparent analytical sensitivity equal or better than the reference real-time or conventional RT-PCR assays and was able to identify mixed virus populations. The ability to identify mixed virus populations at a high analytical sensitivity would be pertinent in the Australian context when attempting to detect exotic JEV or West Nile virus (WNV), and differentiate from endemic Murray Valley encephalitis virus and WNV-Kunjin virus. The relatively low cost, the ability to identify mixed virus populations and the multiplex nature makes this assay valuable for a wide range of applications including diagnostic investigations, virus exclusions, and surveillance programs.
日本脑炎病毒（JEV）血清复合体中的黄病毒包括主要的人类和动物病原体，它们倾向于在新的地理区域传播和出现。这些病毒中的许多已被定义为不同的基因型或遗传谱系，并且分布在全球范围内。能够快速检测新出现的黄病毒并区分重要亚群的工具在虫媒病毒诊断和监测中具有广泛应用，对于检测病毒入侵、追踪病毒出现以及疾病控制至关重要。开发了一种微球悬浮阵列检测方法来鉴定具有医学和兽医学重要性的JEV血清复合体黄病毒。使用代表性病毒株以及临床和监测样本评估了检测性能。该检测方法在本研究中检测到了所有测试的JEV血清复合体病毒，其表观分析灵敏度等于或优于参考实时或传统RT-PCR检测方法，并且能够鉴定混合病毒群体。在澳大利亚试图检测外来JEV或西尼罗河病毒（WNV）并与地方性墨累谷脑炎病毒和WNV-昆金病毒区分开来的情况下，以高分析灵敏度鉴定混合病毒群体的能力将具有相关性。相对较低的成本、鉴定混合病毒群体的能力以及多重性质使得该检测方法对于包括诊断调查、病毒排除和监测计划在内的广泛应用具有价值。
