Wong-Paz Jorge E, Muñiz-Márquez Diana B, Aguilar-Zárate Pedro, Rodríguez-Herrera Raúl, Aguilar Cristóbal N
Department of Food Science and Technology, School of Chemistry, Autonomous University of Coahuila, 25280, Saltillo, Coahuila, Mexico.
Phytochem Anal. 2014 Sep-Oct;25(5):439-44. doi: 10.1002/pca.2512. Epub 2014 Apr 1.
There is increasing interest in phenolic compounds around the world because of their potential positive impact on human health. Phenolic compounds are largely found in fruits and vegetables. Extraction of phenolic compounds is a very important step in their recovery. The newly developed technique of ultrasound-assisted extraction (UAE) appears to be an advantageous alternative compared with conventional techniques, because it is simple and environmental friendly. The potential of UAE needs to be evaluated in each plant in order to demonstrate its efficiency.
The objective of the present study was to compare a conventional method and UAE on the extraction efficiency of phenolic compounds from Jatropha dioica, Fluorensia cernua, Turnera diffusa and Eucalyptus camaldulensis plants and evaluate the in vitro anti-oxidant potential.
Validation of the new method was carried out using mixed-model methodology and regression analysis. Feasibility of this new method was shown and applied using several plants extracts obtained by different extraction methods from semi-arid Mexican plants, which were characterised by high levels of polyphenols. Additionally, the anti-oxidant potential of these extracts was determined by 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity.
Results showed that the new microplate method can be used to determine total phenolic content in plant extracts. Additionally, an alternative extraction method by ultrasound was less efficient compared with the conventional method.
The tested plants are good candidates to obtain nutraceuticals and functional food ingredients.
由于酚类化合物对人类健康具有潜在的积极影响，世界各地对其的关注度日益增加。酚类化合物主要存在于水果和蔬菜中。酚类化合物的提取是其回收利用的一个非常重要的步骤。新开发的超声辅助提取（UAE）技术与传统技术相比似乎是一种更具优势的替代方法，因为它简单且环保。需要对每种植物的超声辅助提取潜力进行评估，以证明其效率。
本研究的目的是比较传统方法和超声辅助提取法对麻疯树、垂花弗洛伦斯花、白花时钟藤和赤桉植物中酚类化合物的提取效率，并评估其体外抗氧化潜力。
使用混合模型方法和回归分析对新方法进行验证。通过从墨西哥半干旱地区富含多酚的植物中采用不同提取方法获得的几种植物提取物，展示并应用了该新方法的可行性。此外，通过1,1 - 二苯基 - 2 - 苦基肼自由基清除活性测定这些提取物的抗氧化潜力。
结果表明，新的微孔板法可用于测定植物提取物中的总酚含量。此外，与传统方法相比，超声辅助提取的替代方法效率较低。
所测试的植物是获取营养保健品和功能性食品成分的良好候选者。
