墨西哥半干旱地区植物提取物的总酚含量、体外抗氧化活性及化学成分

Total phenolic content, in vitro antioxidant activity and chemical composition of plant extracts from semiarid Mexican region.

作者信息

Wong-Paz Jorge E, Contreras-Esquivel Juan C, Rodríguez-Herrera Raúl, Carrillo-Inungaray María L, López Lluvia I, Nevárez-Moorillón Guadalupe V, Aguilar Cristóbal N

机构信息

Laboratory of Bioprocesses and Natural Products, Food Research Department, School of Chemistry, Universidad Autónoma de Coahuila, Saltillo, 25280, México.

Laboratory of Biochemistry, Multidisciplinary Academic Unit-Huasteca, Universidad Autónoma de San Luis Potosí, 79060, Cd. Valles, S.L.P., Mexico.

出版信息

Asian Pac J Trop Med. 2015 Feb;8(2):104-11. doi: 10.1016/S1995-7645(14)60299-6.

DOI:10.1016/S1995-7645(14)60299-6

PMID:25902023

Abstract

OBJECTIVE

To determine the extraction suitable conditions of total phenolic content (TPC) by heat-reflux system, antioxidant activities and HPLC characterization of the aqueous-ethanolic extracts of Jatropha dioica (J. dioica) (Dragon's blood), Flourensia cernua (F. cernua) (Tar bush), Eucalyptus camaldulensis (E. camaldulensis) (Eucalyptus) and Turnera diffusa (T. diffusa) (Damiana).

METHODS

TPC was evaluated by the well-known colorimetric assay using Folin-Ciocalteu reagent. The antioxidant activities were assayed by three methods based on scavenging of DPPH, ABTS and by lipid oxidation inhibition. The chemical composition of the extracts obtained was subject to HPLC analysis.

RESULTS

TPC in the plant extracts ranged from 2.3 to 14.12 mg gallic acid equivalents/g for J. dioica and E. camaldulensis, respectively. The plant extracts of F. cernua, E. camaldulensis and T. diffusa showed similar strong antioxidant activities on scavenging of DPPH and lipid oxidation inhibition. In contrast, J. dioica extracts had lowest potential antioxidant in three assays used. HPLC assay showed the presence of several phenolic compounds in the extracts used.

CONCLUSIONS

The results obtained suggest that F. cernua, E. camaldulensis and T. diffusa are potential sources to obtain bioactive phenolic compounds with high antioxidant properties which can be used in the factories as antioxidant agents or for treatments in diseases.

摘要

目的

确定通过热回流系统提取二色麻风树（龙血树）、垂花弗洛伦斯木（焦油树）、赤桉（桉树）和白花时钟花（达米阿那）水醇提取物中总酚含量（TPC）的适宜条件、抗氧化活性及进行高效液相色谱（HPLC）表征。

方法

采用著名的福林 - 酚试剂比色法评估总酚含量。基于清除二苯基苦味酰基自由基（DPPH）、阳离子自由基（ABTS）和抑制脂质氧化三种方法测定抗氧化活性。对所获提取物的化学成分进行高效液相色谱分析。

结果

二色麻风树和赤桉植物提取物中的总酚含量分别为2.3至14.12毫克没食子酸当量/克。垂花弗洛伦斯木、赤桉和白花时钟花的植物提取物在清除DPPH和抑制脂质氧化方面表现出相似的强抗氧化活性。相比之下，在所用的三种测定中，二色麻风树提取物的潜在抗氧化能力最低。高效液相色谱分析表明所用提取物中存在几种酚类化合物。