Chabra S, Ranjan M, Bhandari R, Kaur T, Aggrawal M, Puri V, Mahajan N, Kaur I P, Puri S, Sobti R C
Centre for Stem Cell & Tissue Engineering, Panjab University, Departments of 3 Biochemistry & 5 Biotechnology , Panjab University.
University Institute of Pharmaceutical Sciences (UIPS), Panjab University , Sector-14, Chandiagarh-160014, India.
J Stem Cells Regen Med. 2011 Oct 30;7(2):75-9. doi: 10.46582/jsrm.0702012. eCollection 2011.
A rapid decline in self-renewability, viability and function, of isolated stem cells are major hurdles in developing cell based therapies. There has been an increasing interest towards identifying a support material for maintaining stem cell features of the isolated cells. Pioneering observations of the present paper, demonstrate functionally diverse potential of Solid Lipid Nanoparticles (SLNs) in deciding the fate & behavior of mouse mesenchymal stem cell. The evidences are provided to show the dual nature of the SLNs for being a scaffold for the stem cell attachment, to retain stemness, and as reagent for inducing stem cell differentiation. Scanning electron microscopic examinations together with expression analysis were used to conform to such observations. Results of the study thus suggest that Solid lipid nanoparticles can be used as a good support material when functionalized to achieve adhesive properties and as a molecular paradigm for studying the adipocytic differentiation. We envisage a new role of SLNs towards regulating stem cell character by orchestrating the structural alignment during preparation of Solid lipid nanoparticles.
分离的干细胞自我更新能力、活力和功能的迅速下降是开发基于细胞的疗法的主要障碍。人们越来越有兴趣寻找一种支持材料来维持分离细胞的干细胞特性。本文的开创性观察表明，固体脂质纳米颗粒（SLNs）在决定小鼠间充质干细胞的命运和行为方面具有功能多样的潜力。提供的证据表明，SLNs具有双重性质，既是干细胞附着的支架以保持干性，又是诱导干细胞分化的试剂。扫描电子显微镜检查和表达分析被用于证实这些观察结果。因此，该研究结果表明，当功能化以实现粘附特性时，固体脂质纳米颗粒可以用作良好的支持材料，并作为研究脂肪细胞分化的分子范例。我们设想SLNs在制备固体脂质纳米颗粒过程中通过协调结构排列来调节干细胞特性方面具有新的作用。
