Terunuma H, Deng X, Nishino N, Watanabe K
Tokyo Clinic ; Southern Tohoku General Hospital ; Biotherapy Institute of Japan.
Biotherapy Institute of Japan.
J Stem Cells Regen Med. 2013 Apr 30;9(1):9-13. doi: 10.46582/jsrm.0901003. eCollection 2013.
Natural killer (NK) cells have been known to enhance the host immune responses against cancer. NK cell number and cytotoxicity in patients with cancer is often low. Therefore, we developed a large-scale ex vivo NK cell expansion method without feeder layers and introduced NK cell-based autologous immune enhancement therapy (AIET). In this paper, we discuss the epidemiological data that show the relationship between NK activity and cancer incidence, monitoring of NK cell number and activity, anti-cancer activities of NK cells in vitro and in vivo and the effects of the combination of expanded NK cells with monoclonal antibody drugs on cancers through antibody-dependent cellular cytotoxicity. Finally, we also present the clinical cases of NK cell-based AIET and the effect of AIET on advanced stage of pancreatic cancer and on various advanced cancers refractory to conventional therapies. NK cell-based AIET might be a useful strategy in the multidisciplinary approach to cancer.
自然杀伤（NK）细胞已知可增强宿主针对癌症的免疫反应。癌症患者体内的NK细胞数量和细胞毒性通常较低。因此，我们开发了一种无需饲养层的大规模体外NK细胞扩增方法，并引入了基于NK细胞的自体免疫增强疗法（AIET）。在本文中，我们讨论了显示NK活性与癌症发病率之间关系的流行病学数据、NK细胞数量和活性的监测、NK细胞在体外和体内的抗癌活性，以及通过抗体依赖性细胞毒性作用，扩增后的NK细胞与单克隆抗体药物联合对癌症的影响。最后，我们还展示了基于NK细胞的AIET的临床病例，以及AIET对晚期胰腺癌和各种对传统疗法难治的晚期癌症的疗效。基于NK细胞的AIET可能是癌症多学科治疗方法中的一种有用策略。
