Noorafshan Ali, Asadi-Golshan Reza, Abdollahifar Mohammad-Amin, Karbalay-Doust Saied
Nutr Neurosci. 2015 Aug;18(6):248-55. doi: 10.1179/1476830514Y.0000000123. Epub 2014 Apr 3.
Sodium metabisulfite as a food preservative can affect the central nervous system. Curcumin, the main ingredient of turmeric has neuroprotective activity. This study was designed to evaluate the effects of sulfite and curcumin on the medial prefrontal cortex (mPFC) using stereological methods.
Thirty rats were randomly divided into five groups. The rats in groups I-V received distilled water, olive oil, curcumin (100 mg/kg/day), sodium metabisulfite (25 mg/kg/day), and sulfite + curcumin, respectively, for 8 weeks. The brains were subjected to the stereological methods. Cavalieri and optical disector techniques were used to estimate the total volume of mPFC and the number of neurons and glial cells. Intersections counting were applied on the thick vertical uniform random sections to estimate the dendrites length, and classify the spines. Non-parametric tests were used to analyze the data.
The mean mPFC volume, neurons number, glia number, dendritic length, and total spines per neuron were 3.7 mm(3), 365,000, 180,000, 1820 µm, and 1700 in distilled water group, respectively. A reduction was observed in the volume of mPFC (∼8%), number of neurons (∼15%), and number of glia (∼14%) in mPFC of the sulfite group compared to the control groups (P < 0.005). Beside, dendritic length per neuron (∼10%) and the total spines per neuron (mainly mushroom spines) (∼25%) were reduced in the sulfite group (P < 0.005).
The sulfite-induced structural changes in mPFC and curcumin had a protective role against the changes in the rats.
焦亚硫酸钠作为一种食品防腐剂可影响中枢神经系统。姜黄素是姜黄的主要成分，具有神经保护活性。本研究旨在采用体视学方法评估亚硫酸盐和姜黄素对内侧前额叶皮质（mPFC）的影响。
30只大鼠随机分为五组。第I - V组大鼠分别接受蒸馏水、橄榄油、姜黄素（100毫克/千克/天）、焦亚硫酸钠（25毫克/千克/天）和亚硫酸盐 + 姜黄素处理，持续8周。对大脑进行体视学方法检测。采用卡瓦列里法和光学分割器技术估计mPFC的总体积以及神经元和神经胶质细胞的数量。在厚的垂直均匀随机切片上进行交点计数以估计树突长度并对棘突进行分类。采用非参数检验分析数据。
蒸馏水组mPFC的平均体积、神经元数量、神经胶质细胞数量、树突长度和每个神经元的棘突总数分别为3.7立方毫米、365,000、180,000、1820微米和1700。与对照组相比，亚硫酸盐组mPFC的体积减少了约8%，神经元数量减少了约15%，神经胶质细胞数量减少了约14%（P < 0.005）。此外，亚硫酸盐组每个神经元的树突长度减少了约10%，每个神经元的棘突总数（主要是蘑菇状棘突）减少了约25%（P < 0.005）。
亚硫酸盐诱导的mPFC结构变化，而姜黄素对大鼠的这些变化具有保护作用。
