Wang X, Meng X, Li H, Liu W, Shen S, Gao Z
Department of Gynecology, Laiwu People's Hospital, 001 Xuehu avenue, Laiwu, 271100, China,
Clin Transl Oncol. 2014 Nov;16(11):954-8. doi: 10.1007/s12094-014-1178-6. Epub 2014 Apr 3.
The present study aimed to investigate the expression level of MicroRNA-25 (miR-25) in epithelial ovarian cancer (EOC) tissue, and examine its relationship with clinicopathological factors and prognosis of patients with EOC.
Expression levels of miR-25 in 86 pairs of EOC tissue and adjacent normal tissue were measured by quantitative real-time reverse-transcription polymerase chain reaction (qRT-PCR). The comparison of the expression level of miR-25 between EOC tissue and adjacent normal tissue was performed using the two-sample Student's t test. The correlation between the expression of miR-25 and clinicopathological characters was assessed with the two-sample Student's t test. The overall survival was analyzed by log-rank test, and survival curves were plotted according to Kaplan-Meier.
The expression level of miR-25 in EOC tissue was significantly higher than in adjacent normal tissue. The miR-25 expression level was significantly positively correlated with tumor stage, histology, and regional lymph node involvement (P < 0.05). Kaplan-Meier analysis showed that patients with higher levels of miR-25 had significantly poorer survival than those with lower expression of this miRNA in patients, with a 6-year overall survival of 15.96 and 45.89 %, respectively, (P = 0.001). In the multivariate Cox proportional hazards analysis, high miR-25 expression was independently associated with poor survival (P = 0.002; HR = 2.119; 95 % CI = 1.568-3.221).
The increased expression of miR-25 is closely related to poor prognosis of EOC, indicating that miR-25 may serve as a predictive biomarker for the prognosis of EOC.
本研究旨在探讨微小RNA-25（miR-25）在上皮性卵巢癌（EOC）组织中的表达水平，并研究其与EOC患者临床病理因素及预后的关系。
采用定量实时逆转录聚合酶链反应（qRT-PCR）检测86对EOC组织及癌旁正常组织中miR-25的表达水平。采用两样本t检验比较EOC组织与癌旁正常组织中miR-25的表达水平。用两样本t检验评估miR-25表达与临床病理特征的相关性。采用对数秩检验分析总生存期，并根据Kaplan-Meier法绘制生存曲线。
EOC组织中miR-25的表达水平显著高于癌旁正常组织。miR-25表达水平与肿瘤分期、组织学类型及区域淋巴结受累显著正相关（P<0.05）。Kaplan-Meier分析显示，miR-25水平较高的患者生存率显著低于该miRNA表达较低的患者，6年总生存率分别为15.96%和45.89%（P=0.001）。在多变量Cox比例风险分析中，miR-25高表达与不良生存独立相关（P=0.002；HR=2.119；95%CI=1.568-3.221）。
miR-25表达增加与EOC预后不良密切相关，提示miR-25可能作为EOC预后的预测生物标志物。
