Lee Soon Dong, Lee Dong Wan
Department of Science Education, Jeju National University, Jeju, 690-756, Republic of Korea,
Antonie Van Leeuwenhoek. 2014 Jun;105(6):1017-23. doi: 10.1007/s10482-014-0161-0. Epub 2014 Apr 3.
A rod-shaped actinobacterium, designated Sco-A25(T), was isolated from a red-coloured layer of scoria (volcanic ash) in the Republic of Korea and subjected to a polyphasic taxonomic characterization. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain Sco-A25(T) is a member of the genus Nocardioides and formed a tight monophyletic unit with the type strain of Nocardioides plantarum (98.7 % gene similarity). LL-Diaminopimelic acid was detected in the cell wall. The predominant menaquinone is MK-8(H4). The polar lipids found were phosphatidylinositol, diphosphatidylglycerol, phosphatidylglycerol, and an unknown phospholipid. The fatty acid profile was represented by large amounts of saturated, unsaturated and iso-branched fatty acids. The DNA G+C content was 71.6 mol %. Genomic DNA similarity between strain Sco-A25(T) and N. plantarum KCTC 9577(T) was 47.8 %. On the basis of the results of phenotypic, genetic and phylogenetic analyses presented here, strain Sco-A25(T) is considered to represent a novel species of the genus Nocardioides, for which the name Nocardioides rubroscoriae sp. nov. is proposed. The type strain is Sco-A25(T) (=KCTC 19805(T) = DSM 23986(T) = NBRC 107916(T)).
从韩国一层红色火山渣（火山灰）中分离出一株杆状放线菌，命名为Sco-A25(T)，并对其进行了多相分类学鉴定。基于16S rRNA基因序列的系统发育分析表明，菌株Sco-A25(T)是诺卡氏菌属的成员，与植物诺卡氏菌模式菌株形成紧密的单系类群（基因相似度为98.7%）。在细胞壁中检测到LL-二氨基庚二酸。主要的甲基萘醌是MK-8(H4)。发现的极性脂类有磷脂酰肌醇、双磷脂酰甘油、磷脂酰甘油和一种未知的磷脂。脂肪酸谱以大量饱和、不饱和和异分支脂肪酸为特征。DNA G+C含量为71.6 mol%。菌株Sco-A25(T)与植物诺卡氏菌KCTC 9577(T)的基因组DNA相似度为47.8%。基于此处呈现的表型、遗传和系统发育分析结果，菌株Sco-A25(T)被认为代表诺卡氏菌属的一个新物种，为此提出新名称诺卡氏菌红色火山渣菌（Nocardioides rubroscoriae sp. nov.）。模式菌株为Sco-A25(T)（=KCTC 19805(T)=DSM 23986(T)=NBRC 107916(T)）。
