Fine Chemical Laboratory, J-OIL MILLS, Inc., 1746 Nakashinden, Fukuroi-city, Shizuoka, Japan.
Fine Chemical Laboratory, J-OIL MILLS, Inc., 1746 Nakashinden, Fukuroi-city, Shizuoka, Japan.
Nutrition. 2014 May;30(5):596-601. doi: 10.1016/j.nut.2013.10.017. Epub 2013 Nov 1.
The bioavailability and absorption of soyasaponins and soyasapogenols remain unclear; therefore, the aim of this study was to evaluate plasma soyasaponin and soyasapogenol concentrations and absorption characteristics.
Sprague-Dawley rats were divided into three groups (n = 6), and each group was orally administered a single dose of each test sample. Blood was collected from the cervical vein and plasma soyasapogenol concentrations were measured. A Caco-2 cell monolayer absorption assay was performed in which both soyasapogenol A and B (10 μmol/L) were added to the apical side, and soyasapogenol concentrations on the apical and basolateral sides were determined. Permeability parameters also were calculated.
Absorption of soyasapogenol B was better than that of group B soyasaponins. The time needed to reach peak soyasapogenol B concentration in rats administered soyasapogenol B was 1 to 3 h, whereas that in rats administered group B soyasaponins was 8 h. The ratio of plasma soyasapogenol A to plasma soyasapogenol B was lower than the ratio in the test samples administered. The apical-to-basolateral absorption of soyasapogenol B in the Caco-2 human colon cancer monolayer cell model (apparent permeability coefficient [Papp], 5.5 × 10(-6) cm/sec) was higher than that of soyasapogenol A (Papp, 1.6 × 10(-6) cm/sec).
These results suggest that bioavailability of soyasapogenols is better than that of corresponding soyasaponins and that of group B soyasaponins is better than that of group A soyasaponins.
大豆皂甙和大豆皂素的生物利用度和吸收仍不清楚；因此，本研究旨在评估血浆大豆皂甙和大豆皂素浓度及吸收特征。
将 Sprague-Dawley 大鼠分为三组（n = 6），每组大鼠给予单一剂量的各测试样品。从颈静脉采集血液并测量血浆大豆皂素浓度。进行 Caco-2 细胞单层吸收测定，在顶侧加入大豆皂素 A 和 B（10 μmol/L），并测定顶侧和基底外侧的大豆皂素浓度。还计算了渗透性参数。
大豆皂素 B 的吸收优于 B 组大豆皂甙。给予大豆皂素 B 的大鼠达到大豆皂素 B 血浆浓度峰值所需的时间为 1 至 3 小时，而给予 B 组大豆皂甙的大鼠则需要 8 小时。给予大鼠的大豆皂素 A 与大豆皂素 B 的血浆比值低于给予大鼠的测试样品中的比值。在 Caco-2 人结肠癌细胞单层细胞模型中（表观渗透系数 [Papp]，5.5×10(-6)cm/sec），大豆皂素 B 的顶侧至基底侧吸收高于大豆皂素 A（Papp，1.6×10(-6)cm/sec）。
这些结果表明，大豆皂素的生物利用度优于相应的大豆皂甙，B 组大豆皂甙的生物利用度优于 A 组大豆皂甙。
