Rupasinghe H P Vasantha, Jackson Chung-Ja C, Poysa Vaino, Di Berardo Christina, Bewley J Derek, Jenkinson Jonathan
Guelph Center for Functional Foods, Laboratory Services, University of Guelph, Guelph, Ontario, N1H 8J7, Canada.
J Agric Food Chem. 2003 Sep 24;51(20):5888-94. doi: 10.1021/jf0343736.
An efficient analytical method utilizing high-performance liquid chromatography (HPLC)/evaporative light scattering detector (ELSD) was developed to isolate and quantify the two major soyasaponin aglycones or precursors in soybeans, triterpene soyasapogenol A and B. Soaking of seeds in water up to 15 h did not change the content of soyasapogenols. Seed germination had no influence on soyasapogenol A content but increased the accumulation of soyasapogenol B. Soyasapogenols were mainly concentrated in the axis of the seeds as compared with the cotyledons and seed coat. In the seedling, the root (radicle) contained the highest concentration of soyasapogenol A, while the plumule had the greatest amounts of soyasapogenol B. In 10 advanced food-grade soybean cultivars grown in four locations in Ontario, total soyasapogenol content in soybeans was 2 +/- 0.3 mg/g. Soyasapogenol B content (1.5 +/- 0.27 mg/g) was 2.5-4.5-fold higher than soyasapogenol A content (0.49 +/- 0.1 mg/g). A significant variation in soyasapogenol content was observed among cultivars and growing locations. There was no significant correlation between the content of soyasapogenols and the total isoflavone aglycones.
开发了一种高效的分析方法，利用高效液相色谱（HPLC）/蒸发光散射检测器（ELSD）来分离和定量大豆中的两种主要大豆皂苷苷元或前体，即三萜大豆皂苷元A和B。将种子在水中浸泡长达15小时不会改变大豆皂苷元的含量。种子发芽对大豆皂苷元A的含量没有影响，但会增加大豆皂苷元B的积累。与子叶和种皮相比，大豆皂苷元主要集中在种子的胚轴中。在幼苗中，根（胚根）中大豆皂苷元A的浓度最高，而胚芽中大豆皂苷元B的含量最高。在安大略省四个地点种植的10个高级食品级大豆品种中，大豆中大豆皂苷元的总含量为2±0.3毫克/克。大豆皂苷元B的含量（1.5±0.27毫克/克）比大豆皂苷元A的含量（0.49±0.1毫克/克）高2.5至4.5倍。在品种和种植地点之间观察到大豆皂苷元含量存在显著差异。大豆皂苷元的含量与总异黄酮苷元之间没有显著相关性。
