Marković Marija, Ivović Vladimir, Stajner Tijana, Djokić Vitomir, Klun Ivana, Bobić Branko, Nikolić Aleksandra, Djurković-Djaković Olgica
National Reference Laboratory for Toxoplasmosis, Institute for Medical Research, University of Belgrade, Dr. Subotića 4, P.O. Box 102, 11129 Belgrade, Serbia.
National Reference Laboratory for Toxoplasmosis, Institute for Medical Research, University of Belgrade, Dr. Subotića 4, P.O. Box 102, 11129 Belgrade, Serbia.
Comp Immunol Microbiol Infect Dis. 2014 May;37(3):173-9. doi: 10.1016/j.cimid.2014.03.001. Epub 2014 Mar 15.
To contribute to the insight into the worldwide population structure of Toxoplasma gondii, we genetically characterized a total of eight strains isolated from intermediate hosts including humans, sheep and pigeons in Serbia. Although parasite DNA was detected in 28.2% (60/213) of the human samples from 162 patients serologically suspected of active toxoplasmosis, as well as in 5/7 seropositive pigeons and in 2/12 seropositive sheep examined, multilocus PCR-RFLP genotyping, using SAG1, 5'SAG2, 3'SAG2, GRA6, 5'GRA7 and 3'GRA7 as markers, was successful in only four human isolates (of which one was isolated from both the bronchoalveolar lavage fluid and blood samples of a single patient), one sheep and three pigeons. Of the eight isolates, five were type II (62.5%), one was type III, one was atypical, and one had a type I allele at GRA6 as the single locus genotyped. Although type II, as elsewhere in Europe, predominated, these results may suggest a higher genetic diversity of T. gondii in Serbia, reflecting local environmental contamination and also the geographical position of the country in South-East Europe.
为深入了解全球弓形虫的种群结构，我们对从塞尔维亚的中间宿主（包括人类、绵羊和鸽子）中分离出的总共8株菌株进行了基因特征分析。尽管在162例血清学疑似活动性弓形虫病患者的28.2%（60/213）的人类样本中检测到了寄生虫DNA，以及在5/7只血清学阳性的鸽子和2/12只血清学阳性的绵羊样本中检测到了寄生虫DNA，但使用SAG1、5'SAG2、3'SAG2、GRA6、5'GRA7和3'GRA7作为标记的多位点PCR-RFLP基因分型仅在4株人类分离株（其中1株是从一名患者的支气管肺泡灌洗液和血液样本中分离得到的）、1只绵羊和3只鸽子中成功进行。在这8株分离株中，5株为II型（62.5%），1株为III型，1株为非典型型，1株在GRA6位点具有I型等位基因，这是唯一进行基因分型的位点。尽管与欧洲其他地方一样，II型占主导地位，但这些结果可能表明塞尔维亚的弓形虫具有更高的遗传多样性，这反映了当地的环境污染以及该国在东南欧的地理位置。
