Microbiology Group, Rowett Institute of Nutrition and Health, Greenburn Road, Bucksburn, Aberdeen, AB21 9SB, UK.
Microbiology Group, Rowett Institute of Nutrition and Health, Greenburn Road, Bucksburn, Aberdeen, AB21 9SB, UK.
Trends Microbiol. 2014 May;22(5):267-74. doi: 10.1016/j.tim.2014.03.001. Epub 2014 Mar 31.
The human intestinal tract is colonised by a complex community of microbes, which can have major impacts on host health. Recent research on the gut microbiota has largely been driven by the advent of modern sequence-based techniques, such as metagenomics. Although these are powerful and valuable tools, they have limitations. Traditional culturing and phylogeny can mitigate some of these limitations, either by expanding reference databases or by assigning functionality to specific microbial lineages. As such, culture and phylogeny will continue to have crucially important roles in human microbiota research, and will be required for the development of novel therapeutics.
人类肠道中定植着复杂的微生物群落，这些微生物对宿主健康有着重大影响。近年来，基于高通量测序的宏基因组学技术极大地推动了肠道微生物组学的研究。尽管这些技术强大且有价值，但它们也存在一定的局限性。传统的培养和系统发育学可以通过扩展参考数据库或赋予特定微生物谱系功能来缓解其中一些局限性。因此，培养和系统发育学将继续在人类微生物组学研究中发挥至关重要的作用，并且对于新型治疗方法的开发也是必不可少的。
