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糙皮侧耳、鲍鱼菇和裂褶菌预处理玉米秸秆对其体外瘤胃发酵的影响。

Effect of Irpex lacteus, Pleurotus ostreatus and Pleurotus cystidiosus pretreatment of corn stover on its improvement of the in vitro rumen fermentation.

机构信息

College of Engineering, China Agricultural University, Haidian District, Beijing, China.

出版信息

J Sci Food Agric. 2018 Aug;98(11):4287-4295. doi: 10.1002/jsfa.8951. Epub 2018 Mar 22.

DOI:10.1002/jsfa.8951
PMID:29427334
Abstract

BACKGROUND

The present work investigated changes in corn stover pretreated with different white rot fungi. Corn stover was inoculated with Irpex lacteus, Pleurotus ostreatus and Pleurotus cystidiosus prior to incubation under solid-state fermentation conditions at 28 °C for 42 days. Changes in the chemical composition, in vitro rumen degradability, lignocellulolytic enzyme activity and multi-scale structure of the corn stover were analysed.

RESULTS

Content of all lignocellulose components decreased to a certain extent after fungal pretreatment. The total gas production of sterilized corn stover treated with I. lacteus for 42 days increased from 200 to 289 mL g organic matter. Moreover, the cellulase activity was highest at the later stage of I. lacteus pretreatment. Multi-scale structural analysis indicated that white rot fungal pretreatment, and in particular that of I. lacteus, increased and enlarged substrate porosity and caused changes in the structure of corn stover.

CONCLUSION

Irpex lacteus pretreatment improved the nutritional value of corn stover as a ruminant feed by degrading both cellulose and acid-insoluble lignin as well as changing the structure of the cell walls. © 2018 Society of Chemical Industry.

摘要

背景

本研究考察了不同白腐真菌预处理玉米秸秆后发生的变化。将玉米秸秆用糙皮侧耳、糙皮香菇和栓菌接种后,在 28℃下进行固态发酵 42 天。分析了玉米秸秆的化学成分、体外瘤胃降解率、木质纤维素酶活性和多尺度结构的变化。

结果

真菌预处理后,所有木质纤维素成分的含量都有一定程度的下降。经过 42 天糙皮侧耳处理的灭菌玉米秸秆的总产气量从 200 毫升增加到 289 毫升 g 有机物。此外,在糙皮侧耳预处理的后期,纤维素酶活性最高。多尺度结构分析表明,白腐真菌预处理,特别是糙皮侧耳的预处理,增加并扩大了基质的孔隙率,并导致玉米秸秆结构发生变化。

结论

糙皮侧耳预处理通过降解纤维素和酸不溶性木质素以及改变细胞壁的结构,提高了玉米秸秆作为反刍动物饲料的营养价值。© 2018 英国化学学会。

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