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非洲爪蟾硒代半胱氨酸tRNA(Ser)Sec基因的转录：一个结合了内部B框和U6小核RNA基因中也存在的上游元件的系统。

Transcription of the Xenopus laevis selenocysteine tRNA(Ser)Sec gene: a system that combines an internal B box and upstream elements also found in U6 snRNA genes.

作者信息

Carbon P, Krol A

机构信息

Laboratoire de Biochimie, Institut de Biologie Moléculaire et Cellulaire du CNRS, Strasbourg, France.

出版信息

EMBO J. 1991 Mar;10(3):599-606. doi: 10.1002/j.1460-2075.1991.tb07987.x.

DOI:10.1002/j.1460-2075.1991.tb07987.x
PMID:2001675
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC452690/
Abstract

The transcription mode of the Xenopus tRNA(Ser)Sec gene by RNA polymerase III was deciphered by injection of mutant templates into Xenopus oocyte nuclei. tRNA(Ser)Sec represents the paradigm of a new class of RNA polymerase III genes combining tRNA and U snRNA gene regulatory elements. Its promoter is tripartite, constituted by two upstream elements, a PSE and a TATA motif that are interchangeable with those of U6 snRNA genes and an internal box B as in other tRNAs. The B box enables the transcription level dependent on the upstream promoter to be increased. Data obtained indicate that U1 snRNA (Pol II) and tRNA(Ser)Sec (Pol III) genes share at least one transcription factor, implying that the border between transcription systems is less tight than expected.

摘要

通过将突变模板注射到非洲爪蟾卵母细胞核中，解析了RNA聚合酶III对非洲爪蟾tRNA(Ser)Sec基因的转录模式。tRNA(Ser)Sec代表了一类新的RNA聚合酶III基因的范例，这类基因结合了tRNA和U snRNA基因调控元件。其启动子由三部分组成，包括两个上游元件，一个近端序列元件（PSE）和一个TATA基序，它们可与U6 snRNA基因的元件互换，以及一个如其他tRNA中的内部B框。B框可使依赖上游启动子的转录水平提高。所获得的数据表明，U1 snRNA（Pol II）和tRNA(Ser)Sec（Pol III）基因至少共享一种转录因子，这意味着转录系统之间的界限比预期的要宽松。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5902/452690/76ef8d4e3f78/emboj00101-0102-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5902/452690/94376bc6b27d/emboj00101-0099-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5902/452690/35a19322b815/emboj00101-0099-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5902/452690/84ca37bb7fa6/emboj00101-0100-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5902/452690/abd78b8386af/emboj00101-0102-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5902/452690/76ef8d4e3f78/emboj00101-0102-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5902/452690/94376bc6b27d/emboj00101-0099-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5902/452690/35a19322b815/emboj00101-0099-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5902/452690/84ca37bb7fa6/emboj00101-0100-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5902/452690/abd78b8386af/emboj00101-0102-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5902/452690/76ef8d4e3f78/emboj00101-0102-b.jpg

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