Department of Physical Organic Chemistry, Radboud University Nijmegen, Institute for Molecules and Materials, Heyendaalseweg 135, 6525 AJ Nijmegen (The Netherlands) http://www.ru.nl/physicalorganicchemistry/
Angew Chem Int Ed Engl. 2014 Jul 28;53(31):8066-9. doi: 10.1002/anie.201402327. Epub 2014 Apr 2.
A wet stamping method to precisely control concentrations of enzymes and inhibitors in place and time inside layered gels is reported. By combining enzymatic reactions such as autocatalysis and inhibition with spatial delivery of components through soft lithographic techniques, a biochemical reaction network capable of recognizing the spatial distribution of an enzyme was constructed. The experimental method can be used to assess fundamental principles of spatiotemporal order formation in chemical reaction networks.
本文报道了一种湿压印方法，可精确控制分层凝胶内部位置和时间的酶和抑制剂浓度。通过将自催化和抑制等酶反应与通过软光刻技术的组件空间传递相结合，构建了能够识别酶空间分布的生化反应网络。该实验方法可用于评估化学反应网络中时空顺序形成的基本原理。
