Lopes Tatyane Duran, Riegel-Vidotti Izabel Cristina, Grein Aline, Tischer Cesar Augusto, Faria-Tischer Paula Cristina de Sousa
Department of Biochemistry and Biotechnology, CCE, State University of Londrina, PO Box 6001, 86051-990 Londrina, PR, Brazil.
Departamento de Química, Universidade Federal do Paraná (UFPR), CxP 19081, CEP 81531-980 Curitiba, PR, Brazil.
Int J Biol Macromol. 2014 Jun;67:401-8. doi: 10.1016/j.ijbiomac.2014.03.047. Epub 2014 Apr 2.
In this study, the effect of the addition of hyaluronic acid (HA) on bacterial cellulose (BC) production, under static conditions was evaluated in terms of the properties of the resulting BC hybrid membranes. HA was added to the fermentation process in three distinct time points: first day (BC-T0), third day (BC-T3) and sixth day (BC-T6). Analyses of FT-IR and CP/MAS (13)C NMR confirmed the presence of HA in bacterial cellulose membranes. The crystal structure, crystallinity index (Ic) surface roughness, thermal stability and hybrophobic/hydrophilic character changed. Membranes with higher roughness were produced with HA added on the first and third day of fermentation process. The surface energy of BC/HA membranes was calculated and more hydrophilic membranes were produced by the addition of HA on the third and sixth day, also resulting in more thermally stable materials. The results demonstrate that bacterial cellulose/hyaluronic acid hybrid membranes can be produced in situ and suggest that HA interacts with the sub-elementary bacterial cellulose fibrils, changing the properties of the membranes. The study and understanding of the factors that affect those properties are of utmost importance for the safe and efficient use of BC as biomaterials in numerous applications, specifically in the biological field.
在本研究中，根据所得细菌纤维素杂化膜的性能，评估了在静态条件下添加透明质酸（HA）对细菌纤维素（BC）生产的影响。在三个不同的时间点将HA添加到发酵过程中：第一天（BC-T0）、第三天（BC-T3）和第六天（BC-T6）。傅里叶变换红外光谱（FT-IR）和交叉极化/魔角旋转（13）C核磁共振（CP/MAS (13)C NMR）分析证实了细菌纤维素膜中存在HA。晶体结构、结晶度指数（Ic）、表面粗糙度、热稳定性和疏水/亲水特性发生了变化。在发酵过程的第一天和第三天添加HA时，会产生粗糙度更高的膜。计算了BC/HA膜的表面能，在第三天和第六天添加HA会产生更亲水的膜，同时也会产生热稳定性更高的材料。结果表明，可以原位制备细菌纤维素/透明质酸杂化膜，并表明HA与亚基本细菌纤维素原纤维相互作用，改变了膜的性能。研究和了解影响这些性能的因素对于在众多应用中，特别是在生物领域安全有效地将BC用作生物材料至关重要。
