Brugnoli Marcello, Mazzini Ilaria, La China Salvatore, De Vero Luciana, Gullo Maria
Unimore Microbial Culture Collection Laboratory, Department of Life Sciences, University of Modena and Reggio Emilia, 42124 Reggio nell'Emilia, Italy.
NBFC-National Biodiversity Future Center, 90133 Palermo, Italy.
Microorganisms. 2023 Jun 5;11(6):1504. doi: 10.3390/microorganisms11061504.
In this study, a co-culture system combining bacterial cellulose (BC) producers and hyaluronic acid (HA) producers was developed for four different combinations. AAB of the genus sp. and LAB of the genus were used to produce BC and HA, respectively. Fourier-transform infrared spectroscopy, scanning electron microscopy, and X-ray diffraction were used to investigate changes in BC-HA composites chemical and morphological structure. Water absorption, uptake, and antibacterial properties were also tested. Outcomes highlighted a higher bacterial cellulose yield and the incorporation of hyaluronic acid into the composite. The presence of hyaluronic acid increased fiber dimension-nearly doubled for some combinations-which led to a decreased crystallinity of the composites. Different results were observed based on the BC producer and HA producer combination. However, water holding capacity (WHC) in all the samples improved with the presence of HA, while water uptake worsened. A thymol-enriched BC-HA composite showed high antibacterial activity against DSM 30083 and DSM 20231. Results could contribute to opening new applications in the cosmetics or pharmaceutical fields.
在本研究中，针对四种不同组合开发了一种将细菌纤维素（BC）生产者和透明质酸（HA）生产者相结合的共培养系统。分别使用 属的醋酸菌和 属的乳酸菌来生产 BC 和 HA。利用傅里叶变换红外光谱、扫描电子显微镜和 X 射线衍射来研究 BC-HA 复合材料化学和形态结构的变化。还测试了吸水性、吸水量和抗菌性能。结果表明细菌纤维素产量更高，且透明质酸被纳入了复合材料中。透明质酸的存在增加了纤维尺寸——某些组合几乎翻倍——这导致复合材料的结晶度降低。根据 BC 生产者和 HA 生产者的组合观察到了不同的结果。然而，所有样品中的持水能力（WHC）随着 HA 的存在而提高，而吸水量则变差。富含百里酚的 BC-HA 复合材料对 DSM 30083 和 DSM 20231 显示出高抗菌活性。这些结果可能有助于在化妆品或制药领域开拓新的应用。
