Thushara Ram M, Hemshekhar Mahadevappa, Paul Manoj, Shanmuga Sundaram Mahalingam, Shankar Rohith L, Kemparaju Kempaiah, Girish Kesturu S
Department of Studies in Biochemistry, University of Mysore, Manasagangothri, Mysore, 570006, Karnataka, India.
J Thromb Thrombolysis. 2014 Oct;38(3):321-30. doi: 10.1007/s11239-014-1056-7.
Recent studies have reported the platelet proapoptotic propensity of plant-derived molecules such as, resveratrol, thymoquinone, andrographolide and gossypol. Meanwhile, there were also reports of phytochemicals such as cinnamtannin B1, which shows antiapoptotic effect towards platelets. Platelets are mainly involved in hemostasis, thrombosis and wound healing. However, altered platelet functions can have serious pathological outcomes that include cardiovascular diseases. Platelets are sensitive to external and internal stimuli including therapeutic and dietary components. The anuclear platelets do undergo apoptosis via mitochondrial pathway. However, exaggerated rate of platelet apoptosis could lead to thrombocytopenia and other bleeding disorders. The present study deals with ameliorative efficacy of crocin on sesamol-induced platelet apoptosis. The antiapoptotic property of crocin and the proapoptotic tendency of sesamol in platelets were previously demonstrated. Therefore, it was interesting to see how these two compounds would interact and wield their effects on human platelets. Crocin effectively inhibited sesamol-induced oxidative stress on platelets, which was evidenced by the measurement of endogenously generated reactive oxygen species, particularly hydrogen peroxide, and changes in thiol levels. Further, crocin abrogated sesamol-induced biochemical events of apoptosis in platelets, which include intracellular calcium mobilization, changes in mitochondrial membrane integrity, cytochrome c release, caspase activity and phosphatidylserine externalization. Even though sesamol has proapoptotic effects on platelets, its anti-platelet activity cannot be neglected. Thus, the study proposes that sesamol could be supplemented with crocin, an approach that could not only abolish the toxic effects of sesamol on platelets, but also enhance the quality of treatment due to their synergistic action.
最近的研究报道了植物来源分子（如白藜芦醇、百里醌、穿心莲内酯和棉酚）的血小板促凋亡倾向。与此同时，也有关于肉桂单宁B1等植物化学物质的报道，其对血小板显示出抗凋亡作用。血小板主要参与止血、血栓形成和伤口愈合。然而，血小板功能改变可能会导致严重的病理后果，包括心血管疾病。血小板对包括治疗成分和饮食成分在内的外部和内部刺激敏感。无核血小板确实会通过线粒体途径发生凋亡。然而，血小板凋亡率过高可能会导致血小板减少症和其他出血性疾病。本研究探讨了藏红花素对芝麻酚诱导的血小板凋亡的改善作用。藏红花素的抗凋亡特性和芝麻酚在血小板中的促凋亡倾向先前已得到证实。因此，观察这两种化合物如何相互作用并对人血小板发挥作用很有意思。藏红花素有效抑制了芝麻酚诱导的血小板氧化应激，这通过对内源性活性氧（特别是过氧化氢）的测量以及硫醇水平的变化得到证明。此外，藏红花素消除了芝麻酚诱导的血小板凋亡的生化事件，包括细胞内钙动员、线粒体膜完整性变化、细胞色素c释放、半胱天冬酶活性和磷脂酰丝氨酸外化。尽管芝麻酚对血小板有促凋亡作用，但其抗血小板活性也不可忽视。因此，该研究提出芝麻酚可以与藏红花素联合使用，这种方法不仅可以消除芝麻酚对血小板的毒性作用，还可以因其协同作用提高治疗效果。
