Sharief M K, Keir G, Thompson E J
Department of Special Chemical Pathology, Institute of Neurology, London, U.K.
J Neuroimmunol. 1989 Jul;23(2):149-56. doi: 10.1016/0165-5728(89)90034-9.
A new, specific and economical method with enhanced sensitivity for rapid detection of IgM oligoclonal bands in unconcentrated cerebrospinal fluid (CSF) is presented. It is based on the use of glutaraldehyde during immunofixation of electrophoresed specimens and utilizes peroxidase-conjugated F(ab')2 fragment of anti-IgM antibody to avoid cross-reactivity with other CSF gamma globulins. It is able to detect 1 ng of IgM banded protein in electrophoresed CSF. Using this method, oligoclonal IgM was mainly detected in inflammatory and infectious conditions of the central nervous system.
本文介绍了一种新的、特异且经济的方法，该方法能增强对未浓缩脑脊液（CSF）中IgM寡克隆带的快速检测灵敏度。它基于在电泳标本免疫固定过程中使用戊二醛，并利用抗IgM抗体的过氧化物酶偶联F(ab')2片段来避免与其他CSFγ球蛋白发生交叉反应。该方法能够检测电泳CSF中1 ng的IgM条带蛋白。使用此方法，寡克隆IgM主要在中枢神经系统的炎症和感染性疾病中被检测到。
