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醋酸纤维素免疫固定法与聚丙烯酰胺凝胶电泳法在检测未浓缩脑脊液中寡克隆带方面的比较。

A comparison of cellulose acetate immunofixation with polyacrylamide gel electrophoresis for the detection of oligoclonal bands in unconcentrated cerebrospinal fluid.

作者信息

Goodland F C, Thompson E J

出版信息

J Clin Pathol. 1983 Nov;36(11):1309-11. doi: 10.1136/jcp.36.11.1309.

DOI:10.1136/jcp.36.11.1309
PMID:6355198
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC498551/
Abstract

Two methods of electrophoresis for the detection of oligoclonal bands in unconcentrated CSF were compared. A sample of 98 routine CSFs yielded 18 positives by polyacrylamide gel electrophoresis (PAGE) while cellulose acetate electrophoresis with immunofixation (CAIF) gave 13 positives (72% of the PAGE findings). Despite the loss of sensitivity the cellulose acetate electrophoresis was easier to interpret and more suited to a routine hospital laboratory.

摘要

比较了两种用于检测未浓缩脑脊液中寡克隆带的电泳方法。98份常规脑脊液样本经聚丙烯酰胺凝胶电泳（PAGE）检测出18份阳性，而免疫固定醋酸纤维素电泳（CAIF）检测出13份阳性（为PAGE检测结果的72%）。尽管灵敏度有所下降，但醋酸纤维素电泳更易于解读，更适合医院常规实验室。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d6f1/498551/917d2f96441d/jclinpath00516-0104-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d6f1/498551/463f5db2a2a4/jclinpath00516-0104-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d6f1/498551/917d2f96441d/jclinpath00516-0104-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d6f1/498551/463f5db2a2a4/jclinpath00516-0104-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d6f1/498551/917d2f96441d/jclinpath00516-0104-b.jpg

相似文献

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A comparison of cellulose acetate immunofixation with polyacrylamide gel electrophoresis for the detection of oligoclonal bands in unconcentrated cerebrospinal fluid.醋酸纤维素免疫固定法与聚丙烯酰胺凝胶电泳法在检测未浓缩脑脊液中寡克隆带方面的比较。
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本文引用的文献

1
DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.圆盘电泳。II. 方法及其在人血清蛋白中的应用。
Ann N Y Acad Sci. 1964 Dec 28;121:404-27. doi: 10.1111/j.1749-6632.1964.tb14213.x.
2
Two agarose electrophoretic systems for demonstration of oligoclonal bands in cerebrospinal fluid compared.比较了两种用于显示脑脊液中寡克隆带的琼脂糖电泳系统。
Clin Chem. 1980 Feb;26(2):343-5.
3
Detection limits for protein after electrophoresis on cellulose acetate.
Ann Clin Biochem. 1982 Mar;19(Pt 2):117-9. doi: 10.1177/000456328201900211.
4
Nitrocellulose immunofixation following agarose electrophoresis in the study of immunoglobulin G subgroups in unconcentrated cerebrospinal fluid.在未浓缩脑脊液中免疫球蛋白G亚组研究中，琼脂糖电泳后进行硝酸纤维素免疫固定。
Clin Chim Acta. 1982 May 20;121(2):231-6. doi: 10.1016/0009-8981(82)90063-8.
5
Antibody synthesis within the central nervous system: comparisons of CSF IgG indices and electrophoresis.中枢神经系统内的抗体合成：脑脊液IgG指数与电泳的比较
J Clin Pathol. 1983 Mar;36(3):312-5. doi: 10.1136/jcp.36.3.312.
6
Microzone electrophoresis of unconcentrated cerebrospinal fluid using cellulose acetate strips and nigrosin dye.使用醋酸纤维素条和苯胺黑染料对未浓缩的脑脊液进行微区电泳。
Am J Clin Pathol. 1971 Jun;55(6):705-12. doi: 10.1093/ajcp/55.6.705.
7
Comparison of electrophoresis on agar gel and agarose gel in the evaluation of gamma-globulin abnormalities in cerebrospinal fluid and serum in multiple sclerosis.琼脂凝胶电泳与琼脂糖凝胶电泳在评估多发性硬化症患者脑脊液和血清中γ-球蛋白异常方面的比较。
Clin Chim Acta. 1973 Jul 27;46(4):383-90. doi: 10.1016/0009-8981(73)90251-9.
8
Electrophoretic examination of cerebrospinal fluid proteins in multiple sclerosis and other neurological diseases.多发性硬化症及其他神经系统疾病中脑脊液蛋白质的电泳检查
Eur Neurol. 1973;9(4):224-41. doi: 10.1159/000114228.
9
Electrophoretic morphology of gamma globulins in cerebrospinal fluid of multiple sclerosis and other diseases of the nervous system.多发性硬化症及其他神经系统疾病患者脑脊液中γ球蛋白的电泳形态
Neurology. 1970 Oct;20(10):982-90. doi: 10.1212/wnl.20.10.982.
10
Immunofixation electrophoretic techniques applied to identification of proteins in serum and cerebrospinal fluid.免疫固定电泳技术应用于血清和脑脊液中蛋白质的鉴定。
Clin Chem. 1976 Aug;22(8):1262-8.