Mehrbod Parvaneh, Ideris Aini, Omar Abdul Rahman, Hair-Bejo Mohd
Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang Selangor, Malaysia.
BMC Complement Altern Med. 2014 Apr 7;14:131. doi: 10.1186/1472-6882-14-131.
Influenza virus is still a severe respiratory disease affecting human and other species. As conventional drugs are not recommended for long time because of side effects and drug resistance occurrence, traditional medication has been focused as alternative remedy. HESA-A is a natural compound from herbal-marine origin. Previous studies have reported the therapeutic properties of HESA-A on psoriasis vulgaris and different types of cancers and we also showed its anti-inflammatory effects against influenza A infection.
This study was designed to investigate the potential properties of HESA-A as prophylaxis or treatment. To investigate the prophylaxis or treatment activities of HESA-A, Madin-Darby Canine Kidney (MDCK) cells were exposed to HESA-A and influenza A virus in different manners of exposure and different time intervals. The results were evaluated by MTT and HA assays.
It was found that HESA-A is much more effective against influenza cytopathic effects when it is applied for prophylaxis and also in concurrent treatment (p ≤ 0.05) but not in post-infection treatment (p ≥ 0.05).
In conclusion, HESA-A is significantly effective against influenza replication in prophylaxis application affecting the virus penetration/adsorption to the cell without any toxic effect on the cell viability.
流感病毒仍是一种影响人类和其他物种的严重呼吸道疾病。由于传统药物存在副作用且会产生耐药性，不建议长期使用，因此传统药物已成为替代疗法的研究重点。HESA - A是一种源自草药 - 海洋的天然化合物。先前的研究报道了HESA - A对寻常型银屑病和不同类型癌症的治疗特性，我们也展示了其对甲型流感感染的抗炎作用。
本研究旨在探究HESA - A作为预防或治疗药物的潜在特性。为研究HESA - A的预防或治疗活性，将犬肾传代细胞（MDCK）以不同的暴露方式和不同的时间间隔暴露于HESA - A和甲型流感病毒中。通过MTT和血凝素（HA）测定评估结果。
研究发现，当HESA - A用于预防以及同时治疗时，其对流感细胞病变效应的效果更佳（p≤0.05），但在感染后治疗中效果不佳（p≥0.05）。
总之，HESA - A在预防应用中对流感病毒复制具有显著效果，可影响病毒对细胞的穿透/吸附，且对细胞活力无任何毒性作用。
