Taniguchi Shiduku, Miyoshi Seika, Tamaoki Daisuke, Yamada Shoko, Tanaka Keiichiro, Uji Yuya, Tanaka Suzumi, Akimitsu Kazuya, Gomi Kenji
Faculty of Agriculture and Gene Research Center, Kagawa University, Miki, Kagawa 761-0795, Japan.
Faculty of Agriculture and Gene Research Center, Kagawa University, Miki, Kagawa 761-0795, Japan.
J Plant Physiol. 2014 May 1;171(8):625-32. doi: 10.1016/j.jplph.2014.01.007. Epub 2014 Mar 20.
Rice is one of the most important crops worldwide, and it is a model for molecular studies of monocotyledonous species, particularly for understanding the molecular mechanisms of plant disease resistance. Jasmonic acid (JA) is an important plant hormone involved in rice-pathogen interactions. In addition, JA-induced volatiles are known to be involved in the rice defense system regulated by JA signaling. In this study, we isolated a JA-induced terpene synthase from rice, and found that it produces two sesquiterpenes; β-elemene and β-bisabolene. Furthermore, β-elemene exhibited significant antifungal activity against Magnaporthe oryzae; however it did not exhibited any antibacterial activity against Xanthomonas oryzae pv. oryzae. JA-induced accumulation of β-elemene was regulated by OsJAZ8, a rice jasmonate ZIM-domain (JAZ) protein that is involved in the JA signaling pathway, suggesting that β-elemene plays an important role in JA-induced resistance, and that it functions as an antifungal compound in rice.
水稻是全球最重要的作物之一，是单子叶植物分子研究的模式植物，尤其有助于理解植物抗病的分子机制。茉莉酸（JA）是参与水稻与病原体相互作用的一种重要植物激素。此外，已知茉莉酸诱导的挥发物参与由茉莉酸信号调控的水稻防御系统。在本研究中，我们从水稻中分离出一种茉莉酸诱导的萜类合酶，发现它可产生两种倍半萜；β-榄香烯和β-红没药烯。此外，β-榄香烯对稻瘟病菌表现出显著的抗真菌活性；然而，它对水稻白叶枯病菌没有任何抗菌活性。茉莉酸诱导的β-榄香烯积累受OsJAZ8调控，OsJAZ8是一种参与茉莉酸信号通路的水稻茉莉酸ZIM结构域（JAZ）蛋白，这表明β-榄香烯在茉莉酸诱导的抗性中起重要作用，并且它在水稻中作为一种抗真菌化合物发挥作用。
