具有B血型活性的大肠杆菌O86 O抗原多糖的结构研究。
Structural studies of the O-antigenic polysaccharide of Escherichia coli O86, which possesses blood-group B activity.
作者信息
Andersson M, Carlin N, Leontein K, Lindquist U, Slettengren K
机构信息
Department of Vaccine Development and Production, National Bacteriological Laboratory, Stockholm, Sweden.
出版信息
Carbohydr Res. 1989 Feb 1;185(2):211-23. doi: 10.1016/0008-6215(89)80036-9.
The O-specific side-chains of the lipopolysaccharide from Escherichia coli O86:K2:H2 have been investigated using n.m.r. spectroscopy, methylation analysis, and specific degradations, and shown to be composed of the pentasaccharide repeating-unit (formula; see text) which represents the biological repeating-unit. The blood-group B activity was confirmed by an enzyme-linked immunosorbent assay.
利用核磁共振光谱、甲基化分析和特异性降解方法，对大肠杆菌O86:K2:H2脂多糖的O-特异性侧链进行了研究，结果表明其由五糖重复单元（化学式；见正文）组成，该重复单元代表生物学重复单元。通过酶联免疫吸附测定法证实了其血型B活性。
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