Mahdy O A, Mousa W M, Abdel-Maogood S Z, Nader S M, Abdel-Radi S
Department of Parasitology, Faculty of Veterinary Medicine, Cairo Univ. Giza, Egypt.
Department of Zoonoses, Faculty of Veterinary Medicine, Cairo Univ. Giza, Egypt.
Helminthologia. 2020 May 23;57(2):83-90. doi: 10.2478/helm-2020-0013. eCollection 2020 Jun 1.
of dogs and of cattle and buffalo are nematode parasites that cause serious economic and public health problems all over the world. This study aims to provide molecular data to identify and distinguish between spp. from dogs, cattle and buffalo in Egypt. Moreover, constructing a phylogeny and phylogenetic relationships among these spp. were performed through an analytic study of ATPase-6, a mitochondrial gene; 12S, small subunit ribosomal RNA gene and ITS-2, the second internal transcribed spacer nuclear ribosomal gene. from cattle and buffalo were found to be almost identical. The ATPase- 6 and 12S regions showed 87.78 % and 90.38 % nucleotide similarity between and , while for the ITS-2 region, only 78.38 % was found. Analysis of the three studied genes revealed that each spp. has distinct molecular characteristics. Moreover, it was revealed that these genes, especially the ITS-2 gene, are useful and sensitive molecular markers for classifying and studying the phylogenetic analysis and relationships among closely related spp. All sequences obtained in this study were registered in the GenBank under the accession numbers: MG214149 -MG214157.
犬、牛和水牛的[寄生虫名称未给出]是线虫寄生虫，在世界各地造成严重的经济和公共卫生问题。本研究旨在提供分子数据，以识别和区分埃及犬、牛和水牛体内的[寄生虫名称未给出]物种。此外，通过对线粒体基因ATPase - 6、小亚基核糖体RNA基因12S和核糖体基因第二内部转录间隔区ITS - 2进行分析研究，构建了这些[寄生虫名称未给出]物种之间的系统发育和系统发育关系。发现牛和水牛体内的[寄生虫名称未给出]几乎相同。ATPase - 6和12S区域在[寄生虫名称未给出]和[寄生虫名称未给出]之间显示出87.78%和90.38%的核苷酸相似性，而对于ITS - 2区域，仅发现78.38%的相似性。对三个研究基因的分析表明，每个[寄生虫名称未给出]物种都有独特的分子特征。此外，还发现这些基因，尤其是ITS - 2基因，是用于对密切相关的[寄生虫名称未给出]物种进行分类、研究系统发育分析和关系的有用且敏感的分子标记。本研究中获得的所有序列已在GenBank中注册，登录号为：MG214149 - MG214157。
