低强度激光辐射对细胞脂质膜的影响。

The effect of MLS laser radiation on cell lipid membrane.

作者信息

Pasternak Kamila, Wróbel Dominika, Nowacka Olga, Pieszyński Ireneusz, Bryszewska Maria, Kujawa Jolanta

机构信息

Department of Medical Rehabilitation, Faculty of Military Medicine, Medical University of Lodz, Poland.

Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, Poland.

出版信息

Ann Agric Environ Med. 2018 Mar 14;25(1):108-113. doi: 10.5604/12321966.1230734. Epub 2017 Feb 11.

DOI:10.5604/12321966.1230734

PMID:29575876

Abstract

INTRODUCTION

Authors of numerous publications have proved the therapeutic effect of laser irradiation on biological material, but the mechanisms at cellular and subcellular level are not yet well understood.

OBJECTIVE

The aim of this study was to assess the effect of laser radiation emitted by the MLS M1 system (Multiwave Locked System) at two wavelengths (808 nm continuous and 905 nm pulsed) on the stability and fluidity of liposomes with a lipid composition similar to that of human erythrocyte membrane or made of phosphatidylocholine.

MATERIAL AND METHODS

Liposomes were exposed to low-energy laser radiation at surface densities 195 mW/cm (frequency 1,000 Hz) and 230 mW/cm (frequency 2,000 Hz). Different doses of radiation energy in the range 0-15 J were applied. The surface energy density was within the range 0.46 - 4.9 J/cm .

RESULTS

The fluidity and stability of liposomes subjected to such irradiation changed depending on the parameters of radiation used.

CONCLUSIONS

Since MLS M1 laser radiation, depending on the parameters used, affects fluidity and stability of liposomes with the lipid content similar to erythrocyte membrane, it may also cause structural and functional changes in cell membranes.

摘要