Llorens J, Tusell J M, Suñol C, Rodríguez-Farré E
Department of Neurochemistry, Consejo Superior de Investigaciones Científicas, Barcelona, Spain.
Neurotoxicol Teratol. 1989 Mar-Apr;11(2):145-51. doi: 10.1016/0892-0362(89)90053-6.
The effects of subconvulsant doses (10, 15, 30 mg/kg) of the organochlorine insecticide gamma-hexachlorocyclohexane (lindane) on the spontaneous activity of male Wistar rats were assessed in an automated wheel-shaped activity monitor in 4 hr sessions. Most of the activity variables defined on the raw data showed statistically significant differences among treatment groups. These data can be interpreted as a lindane-induced change in the temporal distribution of the activity. The multivariate statistical technique principal component analysis was used to obtain a composite variable that measured this change. The variable obtained was found to be well correlated with dose levels and brain concentration of lindane and was used to estimate a minimal effective dose (MED) of 1.85 mg/kg and a upper limit for a minimal effective concentration (MEC) in brain of 0.84 micrograms/g by a log-linear regression approach. The results obtained with this approach further support the conclusion that the behavioral effects of lindane can be significant at exposure levels far below its LD50.
采用自动轮式活动监测仪，在4小时的时段内评估了有机氯杀虫剂γ-六氯环己烷（林丹）的亚惊厥剂量（10、15、30毫克/千克）对雄性Wistar大鼠自发活动的影响。根据原始数据定义的大多数活动变量在各治疗组之间显示出统计学上的显著差异。这些数据可解释为林丹引起的活动时间分布变化。采用多元统计技术主成分分析来获得一个衡量这种变化的复合变量。发现所获得的变量与林丹的剂量水平和脑浓度具有良好的相关性，并通过对数线性回归方法用于估计最小有效剂量（MED）为1.85毫克/千克，脑内最小有效浓度（MEC）的上限为0.84微克/克。用这种方法获得的结果进一步支持了这样的结论，即林丹在远低于其半数致死剂量（LD50）的暴露水平下就可能产生显著的行为效应。
