Pirruccello S J, Collins M, Wilson J E, McManus B M
Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha 68105-1065.
Clin Immunol Immunopathol. 1989 Aug;52(2):341-5. doi: 10.1016/0090-1229(89)90185-2.
Subsets of CD4+ T cells originally identified functionally as suppressor-inducer and helper-inducer populations have recently been reinterpreted as naive and memory maturational states. The subsets can be identified by the surface expression of CD45R and CDw29, respectively. Using two-color flow cytometric analysis, we measured these CD4+ T cell subsets in two samples of cord blood and in 26 healthy children between the ages of 1 and 19 years. As has been reported by others, we observed that the majority of CD4+ T cells in cord blood consist predominantly of the CD45R+ subset. With aging we could demonstrate a gradual acquisition of CDw29+, CD4+ T cells and a concomitant gradual decrease in the percentage of CD45R+, CD4+ T cells. These age-related changes are consistent with the concept of naive (CD45R+) and memory (CDw29+) subsets. Further, because of the dynamic changes, their utilization as prognostic indicators in immunologic disease states cannot be applied to children in the same manner as adults.
最初在功能上被鉴定为抑制诱导细胞群和辅助诱导细胞群的CD4 + T细胞亚群，最近被重新解释为初始和记忆成熟状态。这些亚群可分别通过CD45R和CDw29的表面表达来鉴定。使用双色流式细胞术分析，我们在两份脐带血样本以及26名年龄在1至19岁之间的健康儿童中测量了这些CD4 + T细胞亚群。正如其他人所报道的，我们观察到脐带血中的大多数CD4 + T细胞主要由CD45R +亚群组成。随着年龄增长，我们可以证明CDw29 + CD4 + T细胞逐渐增加，同时CD45R + CD4 + T细胞的百分比逐渐下降。这些与年龄相关的变化与初始（CD45R +）和记忆（CDw29 +）亚群的概念一致。此外，由于这些动态变化，它们作为免疫疾病状态预后指标在儿童中的应用方式与成人不同。
