Wadajkar Aniket S, Ahn Chul, Nguyen Kytai T, Zhu Qiang, Komabayashi Takashi
Department of Bioengineering, University of Texas at Arlington, Arlington, TX 76019, USA ; Joint Biomedical Engineering Program, University of Texas at Arlington and University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
Department of Clinical Sciences, University of Texas Southwestern Medical Center, Dallas, TX 75390-9066, USA.
ISRN Dent. 2014 Mar 3;2014:191068. doi: 10.1155/2014/191068. eCollection 2014.
The aim of this study was to evaluate cytotoxicity of direct pulp capping materials such as Dycal, Life, ProRoot MTA, and Super-Bond C&B on L929 fibroblasts. Freshly mixed or set materials were prepared and eluted by incubation with cell culture medium for working time period (fresh) or for 6 hours (set). The cells were exposed to media containing elutes for 24 hours, after which the cell survival was evaluated by MTS assays. In freshly mixed materials, average ± standard deviation % cell viabilities were 40.2 ± 14.0%, 43.7 ± 16.0%, 72.9 ± 12.7%, and 66.0 ± 13.6% for Dycal, Life, ProRoot MTA, and Super-Bond C&B, respectively. There was no statistical difference in cell viabilities among material groups, whereas in set materials, the cell viabilities were 48.7 ± 14.8%, 37.2 ± 10.6%, 46.7 ± 15.2%, and 100 ± 21.9% for Dycal, Life, ProRoot MTA, and Super-Bond C&B, respectively. Super-Bond C&B showed more cell viabilities than the other three material groups (P < 0.05). The four vital pulp therapy materials had similar cytotoxicity when the materials were fresh. Super-Bond C&B was less cytotoxic than Dycal, Life, and ProRoot MTA after the materials were set, which suggests the use of SB-C&B in future in vivo clinical investigations.
本研究的目的是评估Dycal、Life、ProRoot MTA和Super-Bond C&B等直接盖髓材料对L929成纤维细胞的细胞毒性。制备新鲜混合或凝固的材料，并通过在细胞培养基中孵育工作时间段（新鲜）或6小时（凝固）进行洗脱。将细胞暴露于含有洗脱液的培养基中24小时，之后通过MTS试验评估细胞存活率。在新鲜混合材料中，Dycal、Life、ProRoot MTA和Super-Bond C&B的平均±标准差细胞活力百分比分别为40.2±14.0%、43.7±16.0%、72.9±12.7%和66.0±13.6%。材料组之间的细胞活力没有统计学差异，而在凝固材料中，Dycal、Life、ProRoot MTA和Super-Bond C&B的细胞活力分别为48.7±14.8%、37.2±10.6%、46.7±15.2%和100±21.9%。Super-Bond C&B显示出比其他三个材料组更高的细胞活力（P<0.05）。当材料新鲜时，这四种牙髓治疗材料具有相似的细胞毒性。材料凝固后，Super-Bond C&B的细胞毒性低于Dycal、Life和ProRoot MTA，这表明未来在体内临床研究中可使用SB-C&B。
