Spagnolo Alessandro, Magnin-Robert Maryline, Alayi Tchilabalo Dilezitoko, Cilindre Clara, Schaeffer-Reiss Christine, Van Dorsselaer Alain, Clément Christophe, Larignon Philippe, Ramirez-Suero Montserrat, Chong Julie, Bertsch Christophe, Abou-Mansour Eliane, Fontaine Florence
Phytopathology. 2014 Oct;104(10):1021-35. doi: 10.1094/PHYTO-01-14-0007-R.
Botryosphaeria dieback is a fungal grapevine trunk disease that represents a threat for viticulture worldwide due to the decreased production of affected plants and their premature death. This dieback is characterized by a typical wood discoloration called brown stripe. Herein, a proteome comparison of the brown striped wood from Botryosphaeria dieback-affected standing vines cultivars Chardonnay, Gewurztraminer, and Mourvèdre was performed. The transcript analysis for 15 targeted genes and the quantification of both total phenolics and specific stilbenes were also performed. Several pathogenesis-related proteins and members of the antioxidant system were more abundant in the brown striped wood of the three cultivars, whereas other defense-related proteins were less abundant. Additionally, total phenolics and some specific stilbenes were more accumulated in the brown striped wood. Strongest differences among the cultivars concerned proteins of the primary metabolism, which looked to be particularly impaired in the brown striped wood of 'Chardonnay'. Low abundance of some proteins involved in defense response probably contributes to make global response insufficient to avoid the symptom development. The differential susceptibility of the three grapevine cultivars could be linked to the diverse expression of various proteins involved in defense response, stress tolerance, and metabolism.
葡萄座腔菌枝干溃疡病是一种真菌性葡萄树树干病害，由于受感染植株产量下降及其过早死亡，对全球葡萄栽培构成威胁。这种枝干溃疡病的特征是一种典型的木材变色，称为褐色条纹。在此，对受葡萄座腔菌枝干溃疡病影响的直立葡萄品种霞多丽、琼瑶浆和慕合怀特的褐色条纹木材进行了蛋白质组比较。还对15个目标基因进行了转录分析，并对总酚类物质和特定芪类化合物进行了定量分析。几个病程相关蛋白和抗氧化系统成员在三个品种的褐色条纹木材中含量更高，而其他防御相关蛋白含量较低。此外，总酚类物质和一些特定芪类化合物在褐色条纹木材中积累更多。品种间最显著的差异涉及初级代谢的蛋白质，这些蛋白质在‘霞多丽’的褐色条纹木材中似乎受到特别损害。一些参与防御反应的蛋白质丰度较低，可能导致整体反应不足以避免症状发展。这三个葡萄品种的不同易感性可能与参与防御反应、胁迫耐受性和代谢的各种蛋白质的不同表达有关。
