Fernandez Olivier, Lemaître-Guillier Christelle, Songy Aurélie, Robert-Siegwald Guillaume, Lebrun Marc-Henri, Schmitt-Kopplin Philippe, Larignon Philippe, Adrian Marielle, Fontaine Florence
Unité Résistance Induite et Bioprotection des Plantes EA 4707, USC INRAE 1488, SFR Condorcet FR CNRS 3417, Université de Reims Champagne-Ardenne, 51100 Reims, France.
Agroécologie, Institut Agro Dijon, CNRS, INRAE, Université Bourgogne Franche-Comté, 21000 Dijon, France.
Plants (Basel). 2023 Feb 8;12(4):753. doi: 10.3390/plants12040753.
(1) Background: Grapevine trunk diseases (GTDs) have become a global threat to vineyards worldwide. These diseases share three main common features. First, they are caused by multiple pathogenic micro-organisms. Second, these pathogens often maintain a long latent phase, which makes any research in pathology and symptomatology challenging. Third, a consensus is raising to pinpoint combined abiotic stresses as a key factor contributing to disease symptom expression. (2) Methods: We analyzed the impact of combined abiotic stresses in grapevine cuttings artificially infected by two fungi involved in Botryosphaeria dieback (one of the major GTDs), and . Fungal-infected and control plants were subjected to single or combined abiotic stresses (heat stress, drought stress or both). Disease intensity was monitored thanks to the measurement of necrosis area size. (3) Results and conclusions: Overall, our results suggest that combined stresses might have a stronger impact on disease intensity upon infection by the less virulent pathogen . This conclusion is discussed through the impact on plant physiology using metabolomic and transcriptomic analyses of leaves sampled for the different conditions.
(1) 背景：葡萄树干病害（GTDs）已成为全球葡萄园面临的一大威胁。这些病害具有三个主要共同特征。其一，它们由多种致病微生物引起。其二，这些病原体通常有较长的潜伏期，这使得病理学和症状学方面的任何研究都具有挑战性。其三，越来越多的共识指出，复合非生物胁迫是导致病害症状表现的关键因素。(2) 方法：我们分析了复合非生物胁迫对人工感染两种与葡萄座腔菌属溃疡病（主要的GTDs之一）相关真菌的葡萄插条的影响。对感染真菌的植株和对照植株施加单一或复合非生物胁迫（热胁迫、干旱胁迫或两者皆有）。通过测量坏死面积大小来监测病害严重程度。(3) 结果与结论：总体而言，我们的结果表明，复合胁迫对感染较弱毒力病原体后的病害严重程度可能有更强的影响。利用针对不同条件采集的叶片进行代谢组学和转录组学分析，从对植物生理学的影响方面对这一结论进行了讨论。
