Laboratory of Virology, Department of Microbiology, Immunology and Parasitology, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul (UFRGS), Av. Sarmento Leite 500, Porto Alegre, CEP 90.050-170, Rio Grande do Sul (RS), Brazil.
Laboratory of Virology, Department of Microbiology, Immunology and Parasitology, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul (UFRGS), Av. Sarmento Leite 500, Porto Alegre, CEP 90.050-170, Rio Grande do Sul (RS), Brazil.
Vet Microbiol. 2014 Jun 25;171(1-2):182-8. doi: 10.1016/j.vetmic.2014.03.012. Epub 2014 Mar 25.
Establishment of latent infection within specific tissues in the host is a common biological feature of the herpesviruses. In the case of bovine herpesvirus 2 (BoHV-2), latency is established in neuronal tissues, while bovine herpesvirus 4 (BoHV-4) and ovine herpesvirus 2 (OvHV-2) latent virus targets on cells of the monocytic lineage. This study was conducted in quest of BoHV-2, BoHV-4 and OvHV-2 DNA in two hundred trigeminal ganglia (TG) specimens, derived from one hundred clinically healthy cattle, majority of them naturally infected with bovine herpesvirus 1 (BoHV-1) and bovine herpesvirus 5 (BoHV-5). Total DNA extracted from ganglia was analyzed by polymerase chain reaction (PCR) designed to amplify part of the genes coding for BoHV-2, and BoHV-4 glycoprotein B and, for OvHV-2, the gene coding for phosphoribosylformylglycinamidine synthase-like protein. BoHV-2 DNA was detected in TG samples of two (2%) and BoHV-4 DNA in nine (9%) of the animals, whereas OvHV-2 DNA could not be detected in any of the TG DNA. The two animals in which BoHV-2 DNA was identified were also co-infected with BoHV-1 and BoHV-5. Within the nine animals in which BoHV-4 DNA was detected, six were also co-infected with BoHV-1 and BoHV-5. This report provides for the first time evidence that viral DNA from BoHV-2 and BoHV-4 can be occasionally detected in TG of naturally infected cattle. Likewise, in this report we provided for the first time evidence that the co-infection of cattle with three distinct bovine herpesviruses might be a naturally occurring phenomenon.
在宿主的特定组织中建立潜伏感染是疱疹病毒的共同生物学特征。在牛疱疹病毒 2 型（BoHV-2）的情况下，潜伏发生在神经元组织中，而牛疱疹病毒 4 型（BoHV-4）和绵羊疱疹病毒 2 型（OvHV-2）潜伏病毒的靶标是单核细胞谱系的细胞。本研究旨在寻找 200 个三叉神经节（TG）标本中的 BoHV-2、BoHV-4 和 OvHV-2 DNA，这些标本来自 100 头临床健康的牛，其中大多数牛自然感染了牛疱疹病毒 1 型（BoHV-1）和牛疱疹病毒 5 型（BoHV-5）。从神经节提取的总 DNA 通过聚合酶链反应（PCR）进行分析，该反应设计用于扩增编码 BoHV-2、BoHV-4 糖蛋白 B 的部分基因，以及 OvHV-2 编码磷酸核糖基甲酰胺基甘氨酸合酶样蛋白的基因。在 2 只（2%）动物的 TG 样本中检测到 BoHV-2 DNA，在 9 只（9%）动物的 TG 样本中检测到 BoHV-4 DNA，而在任何 TG DNA 中均未检测到 OvHV-2 DNA。确定存在 BoHV-2 DNA 的两只动物也同时感染了 BoHV-1 和 BoHV-5。在检测到 BoHV-4 DNA 的 9 只动物中，有 6 只还同时感染了 BoHV-1 和 BoHV-5。本报告首次提供证据表明，BoHV-2 和 BoHV-4 的病毒 DNA 偶尔可在自然感染牛的 TG 中检测到。同样，在本报告中，我们首次提供证据表明，牛同时感染三种不同的牛疱疹病毒可能是一种自然发生的现象。
