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2
Evidence of natural interspecific recombinant viruses between bovine alphaherpesviruses 1 and 5.牛α疱疹病毒 1 型和 5 型之间存在自然种间重组病毒的证据。
Virus Res. 2017 Oct 15;242:122-130. doi: 10.1016/j.virusres.2017.09.018. Epub 2017 Sep 28.
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Bovine herpesvirus-1: Genetic diversity of field strains from cattle with respiratory disease, genital, fetal disease and systemic neonatal disease and their relationship to vaccine strains.牛疱疹病毒1型：患有呼吸道疾病、生殖道疾病、胎儿疾病和全身性新生儿疾病的牛的野外毒株的遗传多样性及其与疫苗毒株的关系。
Virus Res. 2016 Sep 2;223:115-21. doi: 10.1016/j.virusres.2016.06.017. Epub 2016 Jun 29.
4
A new method for simultaneous detection and discrimination of Bovine herpesvirus types 1 (BoHV-1) and 5 (BoHV-5) using real time PCR with high resolution melting (HRM) analysis.一种利用实时聚合酶链反应结合高分辨率熔解曲线分析（HRM）同时检测和鉴别牛疱疹病毒1型（BoHV-1）和5型（BoHV-5）的新方法。
J Virol Methods. 2016 Jan;227:14-22. doi: 10.1016/j.jviromet.2015.10.005. Epub 2015 Oct 23.
5
Effect of vaccination against bovine herpesvirus 1 with inactivated gE-negative marker vaccines on the health of dairy cattle herds.使用灭活的gE阴性标记疫苗接种预防牛疱疹病毒1对奶牛群健康的影响。
Prev Vet Med. 2015 Mar 1;118(4):467-76. doi: 10.1016/j.prevetmed.2015.01.014. Epub 2015 Jan 25.
6
First report of isolation and molecular characterization of bubaline herpesvirus 1 (BuHV1) from Argentinean water buffaloes.从阿根廷水牛中分离出牛疱疹病毒1型（BuHV1）并进行分子特征分析的首次报告。
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Detection of bovine herpesvirus 2 and bovine herpesvirus 4 DNA in trigeminal ganglia of naturally infected cattle by polymerase chain reaction.应用聚合酶链反应检测自然感染牛的三叉神经节中的牛疱疹病毒 2 型和牛疱疹病毒 4 型 DNA。
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8
Multiplex PCR followed by restriction length polymorphism analysis for the subtyping of bovine herpesvirus 5 isolates.多重 PCR 结合限制性长度多态性分析对牛疱疹病毒 5 分离株的亚型分型。
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Characterization of interspecific recombinants generated from closely related bovine herpesviruses 1 and 5 through multiple PCR sequencing assays.通过多重PCR测序分析对由密切相关的牛疱疹病毒1型和5型产生的种间重组体进行表征。
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一种用于牛疱疹病毒1型田间分离株快速分型的新分子方法。

A new molecular method for the rapid subtyping of bovine herpesvirus 1 field isolates.

作者信息

Maidana Silvina S, Miño Samuel, Apostolo Romina M, De Stefano Gabriel A, Romera Sonia A

机构信息

Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Ciudad Autónoma de Buenos Aires, Argentina (Maidana).

Instituto Nacional de Tecnología Agropecuaria (INTA), Instituto de Virología e Innovaciones Tecnológicas (IVIT), INTA-CONICET (Miño, De Stefano, Romera).

出版信息

J Vet Diagn Invest. 2020 Jan;32(1):112-117. doi: 10.1177/1040638719898692.

DOI:10.1177/1040638719898692
PMID:32013802
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7003222/
Abstract

Bovine herpesvirus 1 (BoHV-1) causes several clinical syndromes in cattle worldwide. There are 3 subtypes of BoHV-1: 1.1, 1.2a, and 1.2b. Several molecular methods are commonly used in the detection and characterization of BoHV-1. Among them, restriction endonuclease analysis (REA) and single-nucleotide polymorphism (SNP) analysis of the complete viral genome allow classification of BoHV-1 into different subtypes. However, developing countries need simpler and cheaper screening assays for routine testing. We designed a standard multiplex PCR followed by a REA assay allowing straightforward subclassification of all BoHV-1 isolates tested into 1.1, 1.2a, and 1.2b subtypes based on the analysis of fragment length polymorphism. Our standard multiplex PCR-REA was used to analyze 33 field strains of BoHV-1 isolated from various tissues. The assay confirmed the subtype identified previously by REA. In addition, non-polymorphic or undigested fragments were sequenced in order to confirm the mutation affecting the RE III site. Our PCR-REA method is an affordable and rapid test that will subtype all BoHV-1 strains.

摘要

牛疱疹病毒1型（BoHV-1）在全球范围内的牛群中引发多种临床综合征。BoHV-1有3个亚型：1.1、1.2a和1.2b。几种分子方法常用于BoHV-1的检测和特征分析。其中，对完整病毒基因组进行限制性内切酶分析（REA）和单核苷酸多态性（SNP）分析可将BoHV-1分为不同亚型。然而，发展中国家需要更简单、更便宜的筛查检测方法用于常规检测。我们设计了一种标准多重PCR，随后进行REA检测，通过分析片段长度多态性，可直接将所有检测的BoHV-1分离株分为1.1、1.2a和1.2b亚型。我们的标准多重PCR-REA用于分析从各种组织中分离出的33株BoHV-1野毒株。该检测方法证实了先前通过REA鉴定的亚型。此外，对非多态性或未消化的片段进行测序，以确认影响RE III位点的突变。我们的PCR-REA方法是一种经济实惠且快速的检测方法，可对所有BoHV-1毒株进行亚型分类。