Anubala S, Sekar R, Nagaiah K
Natural Product Chemistry Division, CSIR - Indian Institute of Chemical Technology, Hyderabad 500007, India.
Analytical Chemistry Division, CSIR - Indian Institute of Chemical Technology, Hyderabad 500007, India.
Talanta. 2014 Jun;123:10-7. doi: 10.1016/j.talanta.2014.01.017. Epub 2014 Feb 4.
A simple, fast and efficient non-aqueous capillary electrophoresis method (NACE) was developed for the simultaneous determination of three major bioactive curcuminoids (CMNs) in Curcuma longa rhizomes and its herbal products. Good separation, resolution and reproducibility were achieved with the background electrolyte (BGE) consisting a mixture of 15.0 mM sodium tetraborate and 7.4 mM sodium hydroxide (NaOH) in 2:10:15 (v/v/v) of water, 1-propanol, and methanol. The influences of background electrolyte, sodium hydroxide, water, sodium dodecyl sulfate and hydroxylpropyl-β-cyclodextrin on separations were investigated. The separation was carried out in a fused-silica capillary tube with reverse polarity. Hydrodynamic injection of 25mbar for 12s was used for injecting samples and a voltage of 28 kV was applied for separation. The ultrasonication method was used for the extraction of CMNs from the turmeric herbal products and the extract was filtered and directly injected without any further treatments. The limits of detection and quantification were less than 5.0 and 14.6 µg/ml respectively for all CMNs. The percentage recoveries for CMNs were >97.2% (%RSD, <2.62). The results obtained by the method were compared with existing spectrophotometric and HPLC methods. The related compounds in the extract did not interfere in the determination of CMNs. The proposed NACE method is better than existing chromatographic and electrophoretic methods in terms of simple electrophoretic medium, fast analysis and good resolution.
建立了一种简单、快速、高效的非水毛细管电泳法（NACE），用于同时测定姜黄根茎及其草药产品中的三种主要生物活性姜黄素类化合物（CMNs）。以15.0 mM四硼酸钠和7.4 mM氢氧化钠（NaOH）在水、1-丙醇和甲醇体积比为2:10:15（v/v/v）的混合溶液作为背景电解质（BGE），实现了良好的分离度、分辨率和重现性。研究了背景电解质、氢氧化钠、水、十二烷基硫酸钠和羟丙基-β-环糊精对分离的影响。分离在具有反相极性的熔融石英毛细管中进行。采用25mbar压力、12s的流体动力学进样方式进样，施加28 kV电压进行分离。采用超声法从姜黄草药产品中提取CMNs，提取物经过过滤后直接进样，无需进一步处理。所有CMNs的检测限和定量限分别小于5.0和14.6 µg/ml。CMNs的回收率大于97.2%（%RSD，<2.62）。将该方法得到的结果与现有的分光光度法和HPLC法进行比较。提取物中的相关化合物不干扰CMNs的测定。所提出的NACE方法在电泳介质简单、分析快速和分辨率良好方面优于现有的色谱法和电泳法。
