超小超顺磁性氧化铁纳米颗粒对人神经前体细胞的预标记。

Ultrasmall superparamagnetic iron oxide nanoparticle prelabelling of human neural precursor cells.

机构信息

Department of Anatomy and Histology, Bosch Institute, Sydney Medical School, University of Sydney, NSW 2006, Australia.

Department of Anatomy and Histology, Bosch Institute, Sydney Medical School, University of Sydney, NSW 2006, Australia; Biomolecular and Physical Sciences, Griffith University, QLD 4111, Australia.

出版信息

Biomaterials. 2014 Jul;35(21):5549-64. doi: 10.1016/j.biomaterials.2014.03.061. Epub 2014 Apr 13.

DOI:10.1016/j.biomaterials.2014.03.061

PMID:24726535

Abstract

Stem cells prelabelled with iron oxide nanoparticles can be visualised using magnetic resonance imaging (MRI). This technique allows for noninvasive long-term monitoring of migration, integration and stem cell fate following transplantation into living animals. In order to determine biocompatibility, the present study investigated the biological impact of introducing ultrasmall superparamagnetic iron oxide nanoparticles (USPIOs) into primary human fetal neural precursor cells (hNPCs) in vitro. USPIOs with a mean diameter of 10-15 nm maghemite iron oxide core were sterically stabilised by 95% methoxy-poly(ethylene glycol) (MPEG) and either 5% cationic (NH2) end-functionalised, or 5% Rhodamine B end-functionalised, polyacrylamide. The stabilising polymer diblocks were synthesised by reversible addition-fragmentation chain transfer (RAFT) polymerisation. Upon loading, cellular viability, total iron capacity, differentiation, average distance of migration and changes in intracellular calcium ion concentration were measured to determine optimal loading conditions. Taken together we demonstrate that prelabelling of hNPCs with USPIOs has no significant detrimental effect on cell biology and that USPIOs, when utilised at an optimised dosage, are an effective means of noninvasively tracking prelabelled hNPCs.

摘要

经氧化铁纳米颗粒预标记的干细胞可以使用磁共振成像（MRI）进行可视化。该技术允许在将其移植到活体动物后，对迁移、整合和干细胞命运进行非侵入性的长期监测。为了确定生物相容性，本研究在体外研究了将超小超顺磁氧化铁纳米颗粒（USPIO）引入原代人胎儿神经前体细胞（hNPC）中的生物学影响。平均直径为 10-15nm 磁赤铁矿氧化铁核的 USPIO 由 95%甲氧基聚（乙二醇）（MPEG）和 5%阳离子（NH2）端官能化或 5%罗丹明 B 端官能化的聚丙烯酰胺稳定。稳定聚合物二嵌段通过可逆加成-断裂链转移（RAFT）聚合合成。在加载后，测量细胞活力、总铁容量、分化、迁移的平均距离和细胞内钙离子浓度的变化，以确定最佳加载条件。总之，我们证明了用 USPIO 对 hNPC 进行预标记对细胞生物学没有明显的不良影响，并且当以优化的剂量使用时，USPIO 是一种有效的非侵入性追踪预标记 hNPC 的方法。