Volk T, Wang S, Rotstein B, Paululat A
Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel.
Fachbereich Biologie/Chemie, Universität Osnabrück, Barbarastr. 11, D-49069 Osnabrueck, Germany.
Matrix Biol. 2014 Jul;37:162-6. doi: 10.1016/j.matbio.2014.03.006. Epub 2014 Apr 13.
The Drosophila model represents an attractive system in which to study the functional contribution of specific genes to organ development. Within the embryo, the heart tube serves as an informative developmental paradigm to analyze functional aspects of matricellular proteins. Here, we describe two essential extracellular matricellular proteins, Multiplexin (Mp) and Lonely heart (Loh). Each of these proteins contributes to the development and morphogenesis of the heart tube by regulating the activity/localization of essential extracellular proteins. Mp, which is secreted by heart cardioblasts and is specifically distributed in the lumen of the heart tube, binds to the signaling protein Slit, and facilitates its local signaling at the heart's luminal domain. Loh is an ADAMTS-like protein, which serves as an adapter protein to Pericardin (a collagen-like protein), promoting its specific localization at the abluminal domain of the heart tube. We also introduce the Drosophila orthologues of matricellular proteins present in mammals, including Thrombospondin, and SPARC, and discuss a possible role for Teneurins (Ten-A and Ten-M) in the heart. Understanding the role of these proteins provides a novel developmental perspective into the functional contribution of matricellular proteins to organ development.
果蝇模型是一个极具吸引力的系统，可用于研究特定基因对器官发育的功能贡献。在胚胎中，心脏管是一个有用的发育范例，可用于分析基质细胞蛋白的功能方面。在此，我们描述了两种重要的细胞外基质细胞蛋白，多重蛋白（Mp）和孤独之心（Loh）。这些蛋白各自通过调节重要细胞外蛋白的活性/定位，对心脏管的发育和形态发生做出贡献。Mp由心脏成肌细胞分泌，特异性分布在心脏管腔中，它与信号蛋白Slit结合，并促进其在心脏腔域的局部信号传导。Loh是一种类ADAMTS蛋白，作为心包蛋白（一种胶原样蛋白）的衔接蛋白，促进其在心脏管腔外域的特异性定位。我们还介绍了哺乳动物中存在的基质细胞蛋白的果蝇同源物，包括血小板反应蛋白和SPARC，并讨论了腱蛋白（Ten-A和Ten-M）在心脏中的可能作用。了解这些蛋白的作用为基质细胞蛋白对器官发育的功能贡献提供了新的发育视角。
