Jonas L, Schütt C, Neels P, Walzel H, Siegl E
Department of Immunology, Wilhelm Pieck University of Rostock, GDR.
Acta Histochem. 1989;85(2):167-73. doi: 10.1016/S0065-1281(89)80063-7.
We produced a monoclonal antibody against human monocytes (ROMO 1) which was identified to be directed against the monocyte specific glycoprotein CD 14. For electron microscopic studies, this antibody was labelled with colloidal gold. By using a preembedding technique, we investigated the binding and internalization of gold labelled ROMO 1. The binding is highly specific. No other cells than monocytes bind it. Suspensions of monocytes, prepared from human blood, were incubated for 15 to 90 min with the marked antibody. The cells were labelled on the whole surface. Already after an incubation period of 15 min, the internalization of the antibody receptor-complex is demonstrable. This process is similar to that of receptor-mediated endocytosis (RME), described for different ligands such as cell nutrients, growth factors, hormones, and others.
我们制备了一种针对人单核细胞的单克隆抗体（ROMO 1），经鉴定它针对单核细胞特异性糖蛋白CD 14。为进行电子显微镜研究，该抗体用胶体金标记。通过采用包埋前技术，我们研究了金标记的ROMO 1的结合及内化情况。这种结合具有高度特异性。除单核细胞外，没有其他细胞能与之结合。用人血制备的单核细胞悬液与标记抗体孵育15至90分钟。细胞整个表面都被标记。在孵育15分钟后，抗体受体复合物的内化就已可被证明。这个过程类似于针对不同配体（如细胞营养物质、生长因子、激素等）所描述的受体介导的内吞作用（RME）。
