利用计算模型模拟仔猪脑缺氧缺血期间的近红外光谱和磁共振波谱测量。
Simulating NIRS and MRS measurements during cerebral hypoxia-ischaemia in piglets using a computational model.
机构信息
CoMPLEX, University College London, London, UK.
Department of Medical Physics and Bioengineering, University College London, Room 3.18, Malet Place Engineering Building, Gower Street, London, WC1E 6BT, UK.
出版信息
Adv Exp Med Biol. 2014;812:187-194. doi: 10.1007/978-1-4939-0620-8_25.
We present a group analysis of the changes in cerebral haemodynamics, and the oxidation state of cytochrome-c-oxidase measured using broadband near-infrared spectroscopy (NIRS) and intracellular pH measured by phosphorous ((31)P) magnetic resonance spectroscopy (MRS) during and after cerebral hypoxia-ischaemia (HI) in 15 piglets. We use a previously published computational model of cerebral metabolism in the piglet [1] to integrate these measurements and simulate HI. We successfully simulate changes in cellular metabolism including shifts in intracellular pH observed in the piglet brain during HI. In this process, we optimise physiological parameters in the model identified through sensitivity analysis (such as the rate of glucose metabolism and intracellular lactate concentration), to fit simulated and measured data. The model fits the data reasonably and suggests a 20 % drop in glucose consumption, a ~65 % increase in lactate concentration and ~35 % drop in the cerebral metabolic rate of oxygen (CMRO₂) during HI.
我们呈现了一项针对 15 头小猪的研究，该研究通过宽带近红外光谱（NIRS）测量脑血液动力学变化和细胞色素 c 氧化酶的氧化状态，通过磷（31P）磁共振波谱（MRS）测量细胞内 pH 值，以观察脑缺氧缺血（HI）期间和之后的变化。我们使用之前发表的小猪脑代谢计算模型[1]来整合这些测量值并模拟 HI。我们成功模拟了细胞代谢的变化，包括在 HI 期间猪脑内观察到的细胞内 pH 值的变化。在这个过程中，我们通过敏感性分析（如葡萄糖代谢率和细胞内乳酸浓度）优化模型中的生理参数，以拟合模拟和测量数据。模型合理地拟合了数据，并表明在 HI 期间葡萄糖消耗下降 20%，乳酸浓度增加约 65%，脑氧代谢率（CMRO₂）下降约 35%。
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