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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Bereta J, Kurdowska A, Koj A, Hirano T, Kishimoto T, Content J, Fiers W, Van Damme J, Gauldie J

Institute of Molecular Biology, Jagiellonian University, Krakow, Poland.

Int J Biochem. 1989;21(4):361-6. doi: 10.1016/0020-711x(89)90359-5.

Rat hepatocytes were cultured for 2 days in Williams E medium containing 1 microM insulin and dexamethasone. 2. Production of five plasma proteins was determined by electroimmunoassay in the media, and amino acid uptake was measured by [alpha-14C]aminoisobutyric acid accumulation in hepatocytes. 3. Supernatants from rat peritoneal macrophages and IL-6/IFN-beta 2/BSF-2 obtained from four different laboratories similarly stimulated synthesis of fibrinogen, alpha 1-cysteine proteinase inhibitor and alpha 2-macroglobulin, as well as [14C]-accumulation in cultured hepatocytes. 4. It is concluded that IL-6 is the principal hepatocyte stimulating factor responsible for typical features of the acute phase response of liver cells.

将大鼠肝细胞在含有1微摩尔胰岛素和地塞米松的威廉姆斯E培养基中培养2天。2. 通过免疫电泳法测定培养基中五种血浆蛋白的产生，并通过[α-14C]氨基异丁酸在肝细胞中的积累来测量氨基酸摄取。3. 来自四个不同实验室的大鼠腹腔巨噬细胞和IL-6/IFN-β2/BSF-2的上清液同样刺激了纤维蛋白原、α1-半胱氨酸蛋白酶抑制剂和α2-巨球蛋白的合成，以及培养肝细胞中[14C]的积累。4. 得出结论，IL-6是负责肝细胞急性期反应典型特征的主要肝细胞刺激因子。

Bereta J, Kurdowska A, Koj A, Hirano T, Kishimoto T, Content J, Fiers W, Van Damme J, Gauldie J

Institute of Molecular Biology, Jagiellonian University, Krakow, Poland.

Int J Biochem. 1989;21(4):361-6. doi: 10.1016/0020-711x(89)90359-5.

Rat hepatocytes were cultured for 2 days in Williams E medium containing 1 microM insulin and dexamethasone. 2. Production of five plasma proteins was determined by electroimmunoassay in the media, and amino acid uptake was measured by [alpha-14C]aminoisobutyric acid accumulation in hepatocytes. 3. Supernatants from rat peritoneal macrophages and IL-6/IFN-beta 2/BSF-2 obtained from four different laboratories similarly stimulated synthesis of fibrinogen, alpha 1-cysteine proteinase inhibitor and alpha 2-macroglobulin, as well as [14C]-accumulation in cultured hepatocytes. 4. It is concluded that IL-6 is the principal hepatocyte stimulating factor responsible for typical features of the acute phase response of liver cells.

将大鼠肝细胞在含有1微摩尔胰岛素和地塞米松的威廉姆斯E培养基中培养2天。2. 通过免疫电泳法测定培养基中五种血浆蛋白的产生，并通过[α-14C]氨基异丁酸在肝细胞中的积累来测量氨基酸摄取。3. 来自四个不同实验室的大鼠腹腔巨噬细胞和IL-6/IFN-β2/BSF-2的上清液同样刺激了纤维蛋白原、α1-半胱氨酸蛋白酶抑制剂和α2-巨球蛋白的合成，以及培养肝细胞中[14C]的积累。4. 得出结论，IL-6是负责肝细胞急性期反应典型特征的主要肝细胞刺激因子。