Hjetland Reidar, Nilsen Roy M, Grude Nils, Ulvestad Elling
Department of Microbiology, General Hospital of Førde, Førde, Norway.
APMIS. 2014 Nov;122(11):1114-24. doi: 10.1111/apm.12267. Epub 2014 Apr 14.
The aim of this study was to assess the seroprevalence of antibodies to Borrelia burgdorferi sensu lato in a healthy adult population from Sogn and Fjordane county in western Norway by different assays. Sera from 1213 blood donors at four different blood banks were analysed in Enzygnost Lyme link VlsE/IgG (IgG), Enzygnost Borreliosis IgM (IgM), and Immunetics C6 Lyme ELISA kit (C6). Sera showing positive or grey-zone reactivities were further examined with Borrelia-EUROLine-RN-AT IgG blot and Borrelia-EUROLine-RN-AT IgM blot. The seroprevalences were 9.6%, 8.2%, 8.4%, 6.4% and 5.7%, respectively. The seroprevalence for IgG was lower in the eastern part of the county and in owners of pet animals. It was higher in men, and increased with age and number of tick bites. C6 and IgG gave comparable results. IgM only was found in 4.5%, more often in women, did not increase with age, and showed no relationship with geography, and 56.4% were positive in IgM blot. In conclusion, antibodies to B. burgdorferi s.l. are common in blood donors in western Norway. The results may be used for evaluation of predictive values of test results in patients, as well as a basis for test algorithms in the laboratory.
本研究旨在通过不同检测方法评估挪威西部松恩-菲尤拉讷郡健康成年人群中抗伯氏疏螺旋体狭义种抗体的血清流行率。对四个不同血库的1213名献血者的血清进行了Enzygnost Lyme link VlsE/IgG（IgG）、Enzygnost莱姆病IgM（IgM）和Immunetics C6莱姆病ELISA试剂盒（C6）检测。对显示阳性或灰区反应性的血清进一步用伯氏疏螺旋体-EUROLine-RN-AT IgG印迹法和伯氏疏螺旋体-EUROLine-RN-AT IgM印迹法检测。血清流行率分别为9.6%、8.2%、8.4%、6.4%和5.7%。该县东部和宠物主人中IgG的血清流行率较低。男性的血清流行率较高，且随年龄和蜱叮咬次数增加。C6和IgG检测结果相当。仅IgM阳性率为4.5%，女性中更常见，不随年龄增加，与地理位置无关，且56.4%的IgM印迹法检测呈阳性。总之，抗伯氏疏螺旋体狭义种抗体在挪威西部献血者中很常见。这些结果可用于评估患者检测结果的预测价值，以及实验室检测算法的基础。
