Bušová Andrea, Dorko Erik, Rimárová Kvetoslava, Diabelková Jana, Rovenská Tímea, Feketeová Eva, Bereš Matúš, Čellár Róbert, Baranová Zuzana, Kampe Tomáš, Benhatchi Karim
Department of Public Health and Hygiene, Faculty of Medicine, Pavol Jozef Safarik University in Kosice, Kosice, Slovak Republic.
Department of Neurology, Faculty of Medicine, Pavol Jozef Safarik University in Kosice and Louis Pasteur University Hospital, Kosice, Slovak Republic.
Cent Eur J Public Health. 2018 Dec;26 Suppl:S67-S71. doi: 10.21101/cejph.a5442.
Lyme disease (LD) is chronic, multi-system zoonosis transmitted by ticks, and LD aetiological agents are spirochetes of the Borrelia burgdorferi sensu lato complex. The aim of the cross-sectional study was to analyze the LD incidence on the basis of the presence of specific antibodies in the serum of patients in Eastern Slovakia, and to compare the results of serological ELISA and immunoblot assays.
Venous blood with questionnaires was obtained by field sampling of respondents from Eastern Slovakia. Overall, we examined 537 human sera by the ELISA and for confirmation we tested all positive IgG antibodies against the Borrelia immunoblot assay.
Our results confirmed the high serum prevalence of anti-Borrelia antibodies (17.9% for IgG), while the immunoblot seropositive test was confirmed in 69.8% of responders from ELISA IgG positive sera. Positive antibodies of the IgM class were found in 7.6% of the population under study. Most commonly found were antibodies against VlsE (80.2%), p41 (66.7%), p18 (56.3%), p100 (41.7%), p58 (31.3%), and p39 (30.2%).
It should be noted that detection of antibodies against B. burgdorferi s.l. is only an indirect evidence of the presence of this bacterium in the development of clinical signs of LD in humans. Laboratory LD tests should be performed in accordance with valid standards, positive and uncertain results must be confirmed by the Western Blot/Immunoblot assay.
莱姆病（LD）是一种由蜱传播的慢性多系统人畜共患病，其病原体是伯氏疏螺旋体狭义复合群的螺旋体。这项横断面研究的目的是根据斯洛伐克东部患者血清中特异性抗体的存在情况分析莱姆病的发病率，并比较血清学酶联免疫吸附测定（ELISA）和免疫印迹分析的结果。
通过对斯洛伐克东部受访者进行现场采样，采集静脉血并填写问卷。总体而言，我们用ELISA检测了537份人类血清，并通过针对伯氏疏螺旋体的免疫印迹分析对所有阳性IgG抗体进行确认检测。
我们的结果证实了抗伯氏疏螺旋体抗体的血清高流行率（IgG为17.9%），而在ELISA IgG阳性血清的69.8%的应答者中免疫印迹血清学检测呈阳性。在所研究人群中，7.6%的人发现了IgM类阳性抗体。最常见的抗体是针对VlsE（80.2%）、p41（66.7%）、p18（56.3%）、p100（41.7%）、p58（31.3%）和p39（30.2%）的抗体。
应该注意的是，检测抗伯氏疏螺旋体狭义种的抗体只是该细菌在人类莱姆病临床症状发展中存在的间接证据。实验室莱姆病检测应按照有效标准进行，阳性和不确定结果必须通过蛋白质印迹/免疫印迹分析进行确认。
