Sulaiman C T, Thushar K V, Satheesh George, Balachandran Indira
a Phytochemistry Division, Centre for Medicinal Plants Research , Arya Vaidya Sala, Kottakkal , India.
Nat Prod Res. 2014;28(13):1021-4. doi: 10.1080/14786419.2014.905562. Epub 2014 Apr 14.
Phenolic characterisation was carried out on the leaf of three Salacia species such as Salacia chinensis, Salacia fruticosa and Salacia oblonga using liquid chromatography coupled with quadrupole time of flight mass spectrometry equipped with electrospray ionisation interface. The estimation of total phenolics was carried out spectrophotometrically using Folin-Ciocalteu method. HPLC diode-array detection has been used for the preliminary identification of phenolic compounds, and liquid chromatography and mass spectrometry analyses were employed for their characterisation. The fragmentation patterns of the compounds during collision-induced dissociation led to the structural elucidation of the separated compounds.
采用配备电喷雾电离接口的液相色谱-四极杆飞行时间质谱联用技术，对中华五层龙、灌木五层龙和椭圆五层龙这三种五层龙属植物的叶片进行了酚类成分分析。采用福林-西奥尔特法通过分光光度法对总酚进行了测定。利用高效液相色谱二极管阵列检测对酚类化合物进行了初步鉴定，并采用液相色谱和质谱分析对其进行了表征。碰撞诱导解离过程中化合物的碎裂模式有助于对分离出的化合物进行结构解析。
