脂肽生物表面活性剂serratwetin W1在大肠杆菌中的异源生产。
Heterologous production of the lipopeptide biosurfactant serrawettin W1 in Escherichia coli.
作者信息
Thies Stephan, Santiago-Schübel Beatrix, Kovačić Filip, Rosenau Frank, Hausmann Rudolf, Jaeger Karl-Erich
机构信息
Institut für Molekulare Enzymtechnologie, Heinrich-Heine-Universität Düsseldorf and Institut für Bio- und Geowissenschaften IBG-1, Forschungszentrum Jülich, D-52426 Jülich, Germany.
Central Division of Analytical Chemistry ZEA-3: Analytik/Biospec, Forschungszentrum Jülich, D-52426 Jülich, Germany.
出版信息
J Biotechnol. 2014 Jul 10;181:27-30. doi: 10.1016/j.jbiotec.2014.03.037. Epub 2014 Apr 13.
The non-ionic biosurfactant serrawettin W1 is a lipopeptide produced by red-pigmented strains of Serratia marcescens which shows antimicrobial, antitumor and plant protecting properties. Here, we report a simple method for heterologous production of this biosurfactant. S. marcescens strain DSM12481 was identified as a novel serrawettin W1 producer and the respective nonribosomal peptide synthetase gene swrW was cloned and expressed in Escherichia coli BL21 Gold. Chemical analysis of heterologous serrawettin W1 revealed that E. coli mainly produced serrawettin with C10 fatty acids as does S. marcescens. Additionally, serrawettin species with longer fatty acids (C13, C14) were detected in S. marcescens which were absent in E. coli. The expression system described here paves the way for the large scale production of this biotechnologically important biosurfactant.
非离子型生物表面活性剂粘质沙雷氏菌素W1是由红色粘质沙雷氏菌菌株产生的一种脂肽，具有抗菌、抗肿瘤和植物保护特性。在此，我们报道了一种异源生产这种生物表面活性剂的简单方法。粘质沙雷氏菌菌株DSM12481被鉴定为一种新型的粘质沙雷氏菌素W1生产者，其相应的非核糖体肽合成酶基因swrW被克隆并在大肠杆菌BL21 Gold中表达。对异源粘质沙雷氏菌素W1的化学分析表明，大肠杆菌主要产生与粘质沙雷氏菌一样的含C10脂肪酸的粘质沙雷氏菌素。此外，在粘质沙雷氏菌中检测到了含有较长脂肪酸（C13、C14）的粘质沙雷氏菌素种类，而大肠杆菌中不存在这些种类。本文所述的表达系统为大规模生产这种具有重要生物技术意义的生物表面活性剂铺平了道路。
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