化学交换饱和转移（CEST）：一种检测蛋白质行为分子信息的有效工具。

Chemical exchange saturation transfer (CEST): an efficient tool for detecting molecular information on proteins' behaviour.

作者信息

Longo Dario Livio, Di Gregorio Enza, Abategiovanni Riccardo, Ceccon Alberto, Assfalg Michael, Molinari Henriette, Aime Silvio

机构信息

Department of Molecular Biotechnologies and Health Sciences, University of Torino, 10126 Torino, Italy.

出版信息

Analyst. 2014 Jun 7;139(11):2687-90. doi: 10.1039/c4an00346b.

DOI:10.1039/c4an00346b

PMID:24733370

Abstract

A versatile method for assessing protein properties via magnetic resonance imaging (MRI) through chemical exchange saturation transfer (CEST) modality is described. The observed CEST signal changes allow monitoring of protein aggregation processes, protein folding/unfolding steps and interaction with lipid membranes.

摘要

描述了一种通过化学交换饱和转移（CEST）模态利用磁共振成像（MRI）评估蛋白质特性的通用方法。观察到的CEST信号变化可用于监测蛋白质聚集过程、蛋白质折叠/去折叠步骤以及与脂质膜的相互作用。