Imani-Nabiyyi Amin, Sorouraddin Mohammad H
Analytical Chemistry Department, Faculty of Chemistry, University of Tabriz, Tabriz, Iran.
Luminescence. 2014 Dec;29(8):994-1002. doi: 10.1002/bio.2649. Epub 2014 Apr 14.
A simple and highly sensitive chemiluminescence (CL) method is reported for the determination of naphazoline hydrochloride (NH). It was found that the weak CL from the reaction of luminol and KIO4 in an alkaline medium could be highly amplified by cysteine-capped cadmium telluride quantum dots (QDs) and the enhanced CL was effectively quenched by NH and this finding was utilized as a basis for the determination of NH. The QDs were synthesized in aqueous medium and characterized by X-ray diffraction (XRD), transmission electron microscopy (TEM), and UV-vis and photoluminescence spectroscopy. A possible mechanism was proposed for the CL system based on radical identification experiments, along with CL spectrum of the system. The experimental parameters were optimized by the reliable response surface methodology (RSM). Under the optimized experimental conditions, the proposed method allowed the determination of NH over the range of 5.0 × 10(-10) -2.0 × 10(-7) mol/L (r(2) = 0.9993, n = 10). The precision (RSD%) of the method, obtained from five replicate determinations of 2.0 and 150 nmol/L NH, was found to be 1.0% and 1.3%, respectively. The method was successfully applied to the determination of NH in pharmaceutical formulations and human urine and serum samples with results corroborated with the aid of those obtained from a standard method.
报道了一种用于测定盐酸萘甲唑啉（NH）的简单且高灵敏度的化学发光（CL）方法。研究发现，鲁米诺与高碘酸钾在碱性介质中反应产生的微弱化学发光可被半胱氨酸包覆的碲化镉量子点（QDs）高度放大，而增强的化学发光会被NH有效猝灭，这一发现被用作测定NH的基础。量子点在水介质中合成，并通过X射线衍射（XRD）、透射电子显微镜（TEM）以及紫外可见和光致发光光谱进行表征。基于自由基鉴定实验以及该体系的化学发光光谱，提出了该化学发光体系的可能机理。通过可靠的响应面法（RSM）对实验参数进行了优化。在优化的实验条件下，所提出的方法能够测定5.0×10⁻¹⁰ - 2.0×10⁻⁷ mol/L范围内的NH（r² = 0.9993，n = 10）。对2.0和150 nmol/L NH进行五次重复测定得到的该方法的精密度（相对标准偏差%）分别为1.0%和1.3%。该方法成功应用于药物制剂以及人尿液和血清样品中NH的测定，其结果通过标准方法得到的结果得到了证实。
