Nickavar Bahman, Adeli Abrisham, Nickavar Azar
Department of Pharmacognosy, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Aliasghar Childern's Hospital, Iran University of Medical Sciences, Tehran, Iran .
Iran J Pharm Res. 2014 Winter;13(1):127-33.
The present work was designed to study the antioxidant activity and to identify the main active components of the essential oil of ajowan (Trachyspermum copticum) fruit. GC and GC-MS analyses of the essential oil showed the presence of eight compounds. The main constituents of the oil were thymol (43.7%), p-cymene (26.8%), and γ-terpinene (24.9%). The antioxidant and free radical scavenging activities of ajowan oil was evaluated by using ABTS(•+) and β-carotene bleaching assays. The oil exhibited a considerable dose-dependent antioxidant activity. Antioxidant activity guided fractionation of the oil was carried out by TLC-bioautography method based on the DPPH(•) assay to screen and separate the main active constituents. The bioautography screening and fractionation resulted in the separation of the main antioxidant compound which was identified as thymol.
本研究旨在研究阿育吠陀（Trachyspermum copticum）果实精油的抗氧化活性，并鉴定其主要活性成分。对该精油进行气相色谱（GC）和气相色谱-质谱联用（GC-MS）分析，结果显示存在8种化合物。该精油的主要成分是百里香酚（43.7%）、对伞花烃（26.8%）和γ-萜品烯（24.9%）。采用ABTS(•+)和β-胡萝卜素漂白法评估了阿育吠陀油的抗氧化和自由基清除活性。该油表现出显著的剂量依赖性抗氧化活性。基于DPPH(•)测定法，通过薄层色谱-生物自显影法对该油进行抗氧化活性导向分级分离，以筛选和分离主要活性成分。生物自显影筛选和分级分离得到了主要抗氧化化合物，鉴定为百里香酚。
