Omidpanah Sahar, Aliakbari Fereshte, Nabavi Seyed Mohammad, Vazirian Mahdi, Hadjiakhoondi Abbas, Kurepaz-Mahmoodabadi Mahdieh, Manayi Azadeh
Medicinal Plants Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, 1417614411 Tehran, Iran.
Infertility & Reproductive Health Research Center, Shahid Beheshti University of Medical Sciences, 1417614411 Tehran, Iran.
Plants (Basel). 2020 Mar 9;9(3):343. doi: 10.3390/plants9030343.
(Apiaceae) plants have several medicinal and condimentary applications and are considered an aphrodisiac agent in Iranian Traditional Medicine. Thus, the present study aims to evaluate the effects of oil from Iranian plants on the viability of spermatogonial stem cells in vitro. The essential oil of fruits was extracted by hydrodistillation, and the amount of thymol was calculated by a gas-chromatography method. Spermatogonial stem cells were isolated from the testes of mice using enzyme digestion. Real-time polymerase chain reaction (RT-PCR) was applied to assess the gene expressions of promyelocytic leukemia zinc finger protein (), DNA-binding protein inhibitor (), tyrosine-protein kinase (), B-cell lymphoma 2 () and Bcl2-associated X protein (). The number and diameter of colonies were also measured in the treated cells. The amount of thymol in the oil was 130.7 ± 7.6 µg/mL. Flow cytometry analysis showed that 92.8% of all cells expressed stimulated by retinoic acid 8 (), a spermatogonial stem cell marker. Expression of and ID-4 genes significantly increased in the treatment groups, while and decreased, and increased in the presence of essential oil. The numbers and diameters of cells were also improved by the application of the plant oil. These data indicated that monoterpenes from the oil of improved the quality and viability of spermatogonia cells in the cell culture.
伞形科植物有多种药用和调味用途，在伊朗传统医学中被视为一种壮阳剂。因此，本研究旨在评估伊朗某植物的油对精原干细胞体外活力的影响。通过水蒸馏法提取该植物果实的精油，并用气相色谱法计算百里香酚的含量。采用酶消化法从小鼠睾丸中分离精原干细胞。应用实时聚合酶链反应（RT-PCR）评估早幼粒细胞白血病锌指蛋白（）、DNA结合蛋白抑制剂（）、酪氨酸蛋白激酶（）、B细胞淋巴瘤2（）和Bcl2相关X蛋白（）的基因表达。还测量了处理后细胞中集落的数量和直径。该油中百里香酚的含量为130.7±7.6µg/mL。流式细胞术分析表明，所有细胞中有92.8%表达了视黄酸刺激基因8（），这是一种精原干细胞标志物。在处理组中，和ID-4基因的表达显著增加，而和减少，并且在精油存在的情况下增加。应用该植物油后，细胞的数量和直径也有所改善。这些数据表明，该植物油中的单萜类化合物提高了细胞培养中精原细胞的质量和活力。
